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  6. Thermo Scientific™ CaptureSelect™ Biotin Anti-LC-kappa (Murine) Conjugate

Thermo Scientific™ CaptureSelect™ Biotin Anti-LC-kappa (Murine) Conjugate
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Catalog No. p-7180822
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7103152100 100 μg
7103152500 500 μg
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Catalog No. 7103152100 Supplier Thermo Scientific™ Supplier No. 7103152100
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Description

Thermo Scientific™ CaptureSelect™ Biotin Anti-LC-kappa (mur) Conjugate consists of a 13 kDa recombinant single domain antibody fragment (VHH affinity ligand) that specifically binds to the constant domain of kappa light chains of mouse and rat antibodies.

CaptureSelect Biotin Anti-LC-kappa (mur) Conjugate consists of a 13 kDa recombinant single domain antibody fragment (VHH affinity ligand) that specifically binds to the constant domain of kappa light chains of mouse and rat antibodies. The affinity ligand is chemically conjugated to biotin via an appropriate spacer that retains the binding reactivity of the ligand when used in combination with streptavidin-based conjugates or streptavidin pre-coated surfaces.

The CaptureSelect Biotin Anti-LC-kappa (mur) Conjugate allows you to:

  • Detect, quantitate, and characterize all mouse and rat antibody isotypes and subclasses (for example, IgG1 to 3, IgM, and IgA), and all Fab fragments thereof, that contain a kappa light chain; as well as Fab and/or Fab2 fragments obtained after papain or pepsin digestion
  • Avoid cross-binding with IgG from bovine sources such as FCS
  • Screen antibody-antigen interactions—biotin anti-LC-kappa (mur) forms a stable [low k(diss)] complex with mouse and rat antibodies through multivalent binding

Applications for CaptureSelect Biotin Anti-LC-kappa (mur) Conjugate include ELISA, Western blot, Gyrolab™-based immunoassays, and label-free detection platforms such as those based on surface plasmon resonance (Biacore™ and IBIS-MX96 systems) and bio-layer interferometry (ForteBio™ Octet™ systems).

Animal origin statement

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12–15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Specifications

Content And Storage Store at -5°C to -30°C.
Form Liquid
Product Line CaptureSelect
Quantity 100 μg
Target Kappa Light Chain
Type Conjugate
Label or Dye Biotin & Other Haptens
Ligand Type Antibody