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  6. Thermo Scientific™ BS3 (bis(sulfosuccinimidyl)suberate)

Thermo Scientific™ BS3 (bis(sulfosuccinimidyl)suberate)

Catalog No. pi21580
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50 mg
10 x 2 mg
1 g
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PI21580 50 mg
PIA39266 10 x 2 mg
PI21586 1 g
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Catalog No. PI21580 Supplier Thermo Scientific™ Supplier No. 21580
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Description

Crosslink amines to amines with BS3; composed of sulfo-NHS-ester groups separated by an 11.4A, water-soluble spacer arm that is useful for cell-surface protein crosslinking.

Thermo Scientific™ Pierce BS3 (Sulfo-DSS) is bis(sulfosuccinimidyl)suberate, an amine-to-amine crosslinker that is homobifunctional, water-soluble, non-cleavable and membrane impermeable.

Highlights:

  • Crosslink cell-surface proteins before cell lysis and immunoprecipitation
  • Identify receptor-ligand interactions by chemical crosslinking
  • “Fix” protein interactions to allow identification of weak or transient protein interactions
  • Identify near-neighbor protein interactions
  • Protein crosslinking to create bioconjugates via single-step reactions
  • Immobilize proteins onto amine-coated surfaces

Specifications

Chemical Reactivity Amine-Amine
Cleavable No
Molecular Weight (g/mol) 572.43
PEGylated No
Spacer Arm Length 11.4 Å
Content And Storage Upon receipt store desiccated at 4°C.
Cell Permeability No
Shipping Condition Ambient
Product Line Pierce
Labeling Method Chemical Labeling
Crosslinker Type Homobifunctional
Reactive Moiety Sulfo-NHS Ester
Spacer Medium (10 to 30 Å)
Form Powder
Quantity 50 mg
Solubility Water
Format Standard, Single-use, Premium-grade
Water Soluble Yes
Product Type Crosslinker
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Frequently Asked Questions (FAQs)

What is the difference between DSS and BS3 crosslinkers?

BS3 is the water-soluble analogue of DSS (i.e., DSS is the membrane-permeable analog of BS3). DSS is water-insoluble and membrane-permeable, so it can be used for both intracellular and cell surface crosslinking. BS3 is water-soluble and membrane-impermeable, so it can be used for cell-surface crosslinking.
What is the protocol for crosslinking IgG to Dynabeads from the Dynabeads Protein G Immunoprecipitation Kit (Cat. No. 10007D) using DSS (disuccinimidyl suberate)?

For crosslinking the antibody to Protein G Dynabeads, please refer to the crosslinking protocol with BS3 (bis(sulfosuccinimidyl)suberate) or Sulpha-DSS, which is an analogue of DSS. The only difference between these two molecules is that DSS is water insoluble and needs to be solubilized in DMSO before use. You can find the protocol at the following link.
Can you provide the shelf-life for BS3 (bis(sulfosuccinimidyl)suberate)?

BS3 (bis(sulfosuccinimidyl)suberate) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

For Research Use Only. Not for use in diagnostic procedures.