missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Crosslinking, Labeling and Protein Modification
  5. Crosslinking Reagents
  6. Thermo Scientific™ BS3 (bis(sulfosuccinimidyl)suberate)

Thermo Scientific™ BS3 (bis(sulfosuccinimidyl)suberate)

Catalog No. PIA39266
Change view
Click to view available options
Quantity:
50 mg
10 x 2 mg
1 g
3 product options available for selection
Product selection table with 3 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
PIA39266 10 x 2 mg
PI21580 50 mg
PI21586 1 g
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 3 options available.
3 options
Catalog No. PIA39266 Supplier Thermo Scientific™ Supplier No. A39266
Only null left

Description

Crosslink amines to amines with BS3; composed of sulfo-NHS-ester groups separated by an 11.4A, water-soluble spacer arm that is useful for cell-surface protein crosslinking.

Thermo Scientific™ Pierce BS3 (Sulfo-DSS) is bis(sulfosuccinimidyl)suberate, an amine-to-amine crosslinker that is homobifunctional, water-soluble, non-cleavable and membrane impermeable.

  • Reactive groups: sulfo-NHS ester (both ends)
  • Reactive towards: amino groups (primary amines)
  • Amine-reactive Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
  • Water-soluble; compare with DSS
  • Membrane-impermeable, allowing for cell surface labeling
  • High-purity, crystalline reagent can be used to create high-purity crosslinked conjugates

Specifications

Chemical Reactivity Amine-Amine
Cleavable No
Molecular Weight (g/mol) 572.43
PEGylated No
Spacer Arm Length 11.4 Å
Content And Storage Upon receipt store desiccated at 4°C.
Cell Permeability No
Shipping Condition Ambient
Product Line No-Weigh, Pierce
Labeling Method Chemical Labeling
Crosslinker Type Homobifunctional
Reactive Moiety Sulfo-NHS Ester
Spacer Medium (10 to 30 Å)
Form Solid
Quantity 10 x 2 mg
Solubility Water
Format Standard, Single-use, Premium-grade
Water Soluble Yes
Product Type Crosslinker
Show More Show Less

Frequently Asked Questions (FAQs)

What is the protocol for crosslinking IgG to Dynabeads from the Dynabeads Protein G Immunoprecipitation Kit (Cat. No. 10007D) using DSS (disuccinimidyl suberate)?

For crosslinking the antibody to Protein G Dynabeads, please refer to the crosslinking protocol with BS3 (bis(sulfosuccinimidyl)suberate) or Sulpha-DSS, which is an analogue of DSS. The only difference between these two molecules is that DSS is water insoluble and needs to be solubilized in DMSO before use. You can find the protocol at the following link.
Can you provide the shelf-life for BS3 (bis(sulfosuccinimidyl)suberate)?

BS3 (bis(sulfosuccinimidyl)suberate) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.
How does a product with "No-Weigh" format reduce my concern over reagent stability?

The No-Weigh product format, where small amounts of reagent are pre-weighed and packaged separately, prevents the contamination and loss of reagent reactivity over time associated with repetitive opening and closing of a single vial. This format enables use of a fresh vial of reagent each time and eliminates the hassle of weighing out small amounts of reagent. Please view additional information and the video at: https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-labeling-crosslinking/no-weigh-packaging.html
Do you recommend crosslinking reagents for viral capsids (diameter 30 nm)?

Amine-to-amine crosslinkers are a good choice for your application since amino groups are usually abundant on the surface of proteins. Our most popular amine-reactive homobifunctional crosslinker that is water-soluble is BS3 (Cat. No. A39266). This has a moderate-length (11.4 A) linker that is good for linking adjacent proteins. If you need the ability to cleave the linker with sulfhydryl reducing agents I would suggest DTSSP (Cat. No. 21578). If you think you might need an extra-long crosslinker, then BS(PEG)5 (Cat. No. 21581) or BS(PEG)9 (Cat. No. 21582) are alternatives.
I would like to use BS3 (Cat. No. A39266) to crosslink a membrane-bound receptor to its ligand and analyze the resulting complex via Western Blotting. Will the complex remain intact when boiled and run in the presence of SDS?

The crosslinks formed by BS3 are stable to SDS/heat treatment as used for gels and blots.

For Research Use Only. Not for use in diagnostic procedures.