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Axygen™ AxyPrep Mag™ Tissue Genomic DNA Kits
For isolation of genomic DNA from Rodent tail tissues
Supplier: Axygen™ MAGTGDNAS
Description
The AxyPrep MAG Tissue-Blood gDNA Kit utilizes a magnetic beads-based technology to isolate genomic DNA from rodent tail tissues. This protocol provides instruction to extract gDNA from fresh or frozen rodent tails. The protocol is performed in 96-well format and 1.5 mL tubes. Purification begins by the addition of lysis buffer, and Proteinase K to rupture cell membranes and digest protein. Genomic DNA is then immobilized on magnetic particles by the addition of a magnetic binding reagent. This differential binding allows the gDNA to be easily separated from contaminants using a magnetic field. Contaminants can then be rinsed away using a simple washing procedure.
- Expanded protocol for animal tissue, cultured cells, buccal swabs, and blood
Specifications
| 96-well Plate, Tube | |
| 33 mL TB1 Buffer, 30 mL TB2 Buffer, 22 mL Wash Buffer, 1.1 mL Proteinase K solution, 1.1 mL FB particles, 30 mL Elution Buffer, 1 protocol | |
| Kit |
| Isolation of genomic DNA from Rodent tail tissues | |
| 48 Preps |