Axygen™ AxyPrep Mag™ FragmentSelect Kits

Post DNA shearing, the library construction process for next generation sequencing mostly requires fragment selection regardless of the platform. Obtaining high recovery post fragment selection is becoming an important contributor for the reduction of sequencing bias. The AxyPrep FragmentSelect-I kit is optimized for Illumina next generation sequencing platforms and Life Technologies (SOLiD) fragment size selection needs, simplifying the library construction workflows. Where, the AxyPrep FragmentSelect-R is designed for fragment size selection needs in the Roche/454s DNA Genome sequencer library construction workflow. The AxyPrep Mag FragmentSelect kits utilize a unique paramagnetic bead technology for quick high-throughput optimized DNA size selection suitable for various next generation sequencing platforms.

• Optimized fragment selection chemistries for Illumina, SOLiD and Roche/454
• Versatile and flexible sequential fragment size selection: no gels needed
• Efficient removal of primer-dimers; no need for further clean-up steps
• Manual and automation friendly
• Scalable: Tube, 96- and 384-well microplate formats
• No centrifugation or filtration required

• 1. Optimization of AxyPrep FragmentSelect beads ratio to input DNA for desired target DNA fragment size selection
• 2. The optimal AxyPrep FragmentSelect beads ratio is utilized for the isolation of the desired target DNA fragment size