MilliporeSigma™ Supelco™ Ascentis™ Express F5 HPLC Column, 5 μm Particle Size

Ascentis™ Express 5 μm F5 is a high-speed, high-performance liquid chromatography column based on the highly efficient Fused-Core™ particle design. The bonded, endcapped, dimethylpentafluorophenyl-propylsilane stationary phase of Ascentis™ Express 5 μm F5 provides a stable, reversed-phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines. In addition to forming π-π and mildly steric interactions, F5 phases also retain compounds by polar interactions. As a result of having both polar and non-polar character, F5 phases can show dual-mode retention behavior, sometimes producing a “U-shaped” retention as a function of acetonitrile content of the mobile phase, with retention increasing at both low and high concentrations of ACN (reversed-phase and HILIC retention modes).