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MilliporeSigma™ Supelco™ Ascentis™ Express F5 HPLC Column, 5 μm Particle Size
Description
Ascentis™ Express 5 μm F5 is a high-speed, high-performance liquid chromatography column based on the highly efficient Fused-Core™ particle design. The bonded, endcapped, dimethylpentafluorophenyl-propylsilane stationary phase of Ascentis™ Express 5 μm F5 provides a stable, reversed-phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines. In addition to forming π-π and mildly steric interactions, F5 phases also retain compounds by polar interactions. As a result of having both polar and non-polar character, F5 phases can show dual-mode retention behavior, sometimes producing a “U-shaped” retention as a function of acetonitrile content of the mobile phase, with retention increasing at both low and high concentrations of ACN (reversed-phase and HILIC retention modes).
Specifications
Specifications
| Diameter (Metric) | 2.1 mm |
| Length (Metric) | 2 cm |
| Particle Size | 5 μm |
| pH | 1 to 9 |
| Pore Size | 90 Å |
| Stationary Phase | PFP (Pentafluorophenyl) |
| Product Line | Ascentis |
| Matrix | PFP (Pentafluorophenyl) Phase/Fused-Core Particle Platform |
| Endcapped | Yes |
| Phase | Reversed Phase |
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Ascentis is a registered trademark of Sigma-Aldrich Co. LLC. Fused-Core is a registered trademark of Advanced Materials Technology, Inc.