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  7. Applied Biosystems™ TaqPath™ ProAmp™ Master Mix

Applied Biosystems™ TaqPath™ ProAmp™ Master Mix

Catalog No. a30871
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Description

TaqPath ProAmp Master Mix is for high-throughput genotyping and copy number variation protocols requiring uncompromising reliability and accuracy, even in the presence of inhibitors commonly found in clinical samples.

TaqPath™ ProAmp™ Master Mix

qPCR master mix for multiplex SNP genotyping and purified DNA–based CNV analysis

TaqPath™ ProAmp™ Master Mix is a qPCR master mix designed for high-throughput multiplex SNP genotyping from purified genomic DNA or crude lysates and for copy number variation (CNV) analysis using purified genomic DNA. The formulation delivers sensitive, reproducible amplification performance even in the presence of PCR inhibitors commonly found in biological samples such as blood, buccal swabs, and card punches.

For SNP genotyping applications, TaqPath ProAmp Master Mix supports accurate and reproducible results using either purified genomic DNA or crude lysate sample inputs. For copy number variation analysis, purified genomic DNA is required.

Manufactured under a robust ISO 13485 quality management system, TaqPath ProAmp Master Mix is designed to deliver consistent lot-to-lot performance for high-throughput and regulated research environments.

Designed for multiplex SNP genotyping

A TaqMan™ SNP Genotyping Assay targets a single SNP locus (a specific genomic position) and includes two PCR primers and two allele-specific probes. Each probe hybridizes to the same genomic region but differs by one nucleotide, allowing detection of one of the two possible alleles at that locus. This design enables allelic discrimination within a single qPCR reaction.

TaqPath™ ProAmp™ Master Mix supports singleplex and multiplex SNP genotyping, including 2-SNP multiplex configurations (two SNP loci per reaction; four allele-specific probes total). This multiplexing approach increases data output per sample while maintaining confident allelic discrimination and assay performance.

Six SNP loci per reaction multiplexing requires a custom TaqPath ProAmp formulation without ROX.

Benefits of TaqPath™ ProAmp™ Master Mix

  • Exceptional data quality—high specificity, reproducibility, and dynamic range* for SNP genotyping and CNV analysis
  • Inhibitor tolerance—robust performance with samples derived from blood, buccal swabs, and card punches
  • Optimized for SNP genotyping from crude lysates—supports accurate SNP genotyping without DNA purification
  • 72-hour pre-PCR benchtop stability with purified DNA—ideal for automated and high-throughput workflows, enabled by the proprietary Dual-Lock™ Taq DNA hot-start mechanism
  • Multiplex flexibility—supports singleplex through four-target SNP detection per reaction
  • Manufacturing consistency—produced in an ISO 13485-certified facility
  • Compliance Package available—includes commonly requested quality and regulatory documentation

Accurate SNP genotyping from purified DNA or crude lysates

TaqPath™ ProAmp™ Master Mix delivers accurate and reproducible SNP genotyping results from either purified genomic DNA or crude lysate inputs. When combined with the DNA Extract All™ Reagents Kit, the master mix supports robust singleplex and 2-locus multiplex SNP genotyping directly from crude lysates prepared from blood and buccal swabs.

Internal testing demonstrated 100% concordance between SNP genotype calls obtained from crude lysates and purified genomic DNA using selected 2-SNP multiplex assay configurations, confirming reliable allelic discrimination without DNA purification.

Accurate SNP genotyping from crude lysates with improved performance versus alternative master mixes

In internal evaluations using TaqMan™ SNP Genotyping Assays and crude blood lysates, TaqPath™ ProAmp™ Master Mix demonstrated improved SNP genotype call accuracy and clearer allelic discrimination compared to selected alternative qPCR master mixes. The formulation produced tight genotype cluster separation and consistent SNP locus calls across replicates, supporting confident genotyping performance even in the presence of inhibitors commonly found in biological samples.

High-accuracy copy number variation (CNV) analysis

In addition to SNP genotyping, TaqPath™ ProAmp™ Master Mix supports accurate copy number variation (CNV) analysis when used with purified genomic DNA. Crude lysate sample inputs are not recommended for CNV analysis.

The formulation enables reliable copy number determination across multiple gene targets, including CYP2D6 intron 2 and CYP2D6 intron 6, with accurate quantification in samples containing one to four copies. Internal comparisons demonstrated improved copy number precision and reproducibility compared to selected alternative qPCR master mixes when purified genomic DNA was used as the input.

Part of a streamlined Sample-to-Genotype workflow

TaqPath™ ProAmp™ Master Mix integrates seamlessly into a complete Sample-to-Genotype workflow, including:

  • DNA Extract All™ Reagents Kit for rapid preparation of crude lysates for SNP genotyping
  • TaqMan™ Predesigned SNP Genotyping Assays or TaqMan™ DME Genotyping Assays
  • Purified genomic DNA for copy number variation analysis
  • QuantStudio™ Real-Time PCR Instruments
  • Diomni™ Design and Analysis Software for multiplex genotyping data analysis

This workflow enables faster turnaround and reduced hands-on time for SNP genotyping, while supporting accurate CNV analysis using purified DNA when required.

General purpose reagents manufactured under cGMP

TaqPath™ master mixes are general purpose reagents labeled For Laboratory Use and are manufactured in an ISO 13485-certified and FDA-registered facility that adheres to current Good Manufacturing Practice (cGMP) principles.

Compliance Package

Frequently requested compliance documents are available, including:

  • Statement of Quality describing adherence to cGMP
  • Lot-specific Certificate of Analysis (COA)
  • Product Stability Statement
  • Supplier Evaluation Support documentation
  • Completed Self Assessment
  • ISO certificates by manufacturing site
  • BSE/TSE Statement
  • Change Control and Notification Registration

For EU REACH compliance-related inquiries, email LSGChemicalCompliance@thermofisher.com.

Not finding the document you need? Technical Support is available to help.

* Dynamic range depends on assay design, template concentration, and master mix formulation; individual results may vary.

Specifications

Content And Storage Store at 2°C to 8°C in the dark.
Detection Method Primer-probe
PCR Method qPCR
Polymerase Taq DNA Polymerase
Reaction Speed Fast or Standard
For Use With (Equipment) 7500 Fast System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 7 Flex, ViiA 7 System, ViiA 7 Dx, QuantStudio Dx, StepOnePlus System, StepOne System
Multiplex Capability 4-plex
Passive Reference Dye ROX (Pre-mixed)
Product Line TaqPath
Product Type qPCR Master Mix
Quantity 2 x 10 mL
Shipping Condition Wet Ice
Sufficient For 4000 Reactions
Target Specificity cDNA or gDNA only
For Use With (Application) Genotyping
Thermostability 72 hr., Assembled Reaction
No. of Reactions 4000 Reactions
Volume 2 x 10 mL
Includes dUTP, UNG/UDG
Inhibitor Tolerance (Simple) Very High
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Frequently Asked Questions (FAQs)

What Applied Biosystems reagents and instruments can I use with TaqMan SNP Genotyping Assays?

The TaqPath ProAmp Master Mix or TaqPath ProAmp Multiplex Master Mix is recommended for TaqMan SNP Genotyping Assays. Additional PCR master mixes that can be used as well, include the TaqMan Universal PCR Master Mix, No AmpErase, TaqMan Universal PCR Master Mix, TaqMan Universal Master Mix II, TaqMan genotyping Master mix, and GTXpress Master Mix. The TaqMan SNP Genotyping Assays can be used on all the real-time PCR instruments from Applied Biosystems, including 7000, 7300, 7500, 7500 Fast, 7700, StepOne, StepOnePlus, 7900HT, ViiA7, and QuantStudio systems.

What is the passive reference dye in the TaqPath ProAmp Master Mix?

The TaqPath ProAmp Master Mix contains ROX dye as the passive reference dye.
The amplification plot shows a high level of noise with TaqPath ProAmp Master Mix (Cat. No. A30865). What can cause this?

This could be caused by selecting an incorrect dye as a passive reference. The TaqPath ProAmp Master Mix contains ROX as a passive reference dye. It needs to be confirmed that ROX has been selected rather than MUSTANG PURPLE, which is present in the TaqPath ProAmp Multiplex Master Mix.
Why am I getting unexpected results for genotyping experiments using crude lysates with the TaqPath ProAmp Master Mix (Cat. No. A30865)?

The assay may not be compatible with a crude lysate. We recommend that you use purified genomic DNA of high quality.
I see small particles in the reaction mix of the TaqPAth ProAmp Master Mix (Cat. No. A30865). Will this cause my reaction to fail?

The small particles seen in the reaction are components of the TaqPath ProAmp Master Mix; they do not interfere with the reaction and do not affect the physical apparatus, including robotics.
Does the TaqPath ProAmp Master Mix (Cat. No. A30865) contain UDG/UNG and/or dUTP?

The TaqPath ProAmp Master Mixes contain both dUTP as well as UDG/UNG enzyme for preventing carry-over contamination.
Which enzyme/polymerase is used in the TaqPath ProAmp Master Mix (Cat. No. A30865)?

The enzyme used in the TaqPath ProAmp Master Mix is the Dual-Lock Taq DNA Polymerase.
Has the TaqPath ProAmp Master Mix (Cat. No. A30865) been tested with Fast mode/cycling using a standard 96-well 0.2 mL block?

The TaqPath ProAmp Master Mix has been tested in the Fast as well as standard mode on a 96-well 0.2 mL block. No difference in results is to be expected.
Is there a specific protocol for preparing the lysate to be used with TaqPath ProAmp Mastermix (Cat. No. A30865)?

The standard protocols described in the user guides of the DNA Extract All Reagent Kit and MagMAX DNA Multi-Sample Ultra Kit should be followed.
Has the tolerance of the TaqPath ProAmp Master Mix (Cat. No. A30865) to the presence of PCR inhibitors been tested?

Hematin, heparin, IgG, EDTA and citrate have been tested as inhibitors of PCR with the TaqPath ProAmp Master Mix.
Have you tested the TaqPath ProAmp Master Mix (Cat. No. A30865) on any other type of sample besides blood, dried blood on filter paper, or buccal swabs?

Besides blood, dried blood on filter paper, and buccal swabs, we have tested the TaqPath ProAmp Master Mix on mouse tails, and it worked well in this in-house testing.
Can the TaqPath ProAmp Master Mix with ROX (Cat. No. A30865) be used on the 7900HT instrument? Which thermal cycling conditions should be used?

Yes, the TaqPath ProAmp Master Mix can be used on the 7900 HT instrument. Please see the complete protocol here.
Can I use TaqPath ProAmp Master Mix (Cat. No. A30865) for CastPCR/TaqMan Mutation Detection Assays?

We have not tested the TaqPath ProAmp Master Mix for use with CastPCR/TaqMan Mutation Detection Assays. The TaqMan Genotyping Master Mix should be used for CastPCR.
What is the initial denaturation/enzyme activation time in the Genotyping run method and the copy number variation protocol using the TaqPath ProAmp Master Mix (Cat. No. A30865)?

The activation time for Genotyping protocol with the TaqPath ProAmp Master Mix is 5 minutes. Copy Number Variation (CNV) assays have a 10-minute activation time. It also ensures that the genomic DNA is fully denatured prior to the cycling stage. Please see the complete protocol here.
What are the benefits of TaqPath ProAmp Master Mixes (Cat. No. A30865)?

The benefits of the TaqPath ProAmp Master Mix are as follows:

  • TaqPath is manufactured in an ISO 13485-certified facility and labeled “For Laboratory Use”.
  • TaqPath raw materials, processes, and specifications are tighter than RUO reagents - giving more confidence in lot-to-lot consistency.
  • 72 hours pre-PCR benchtop stability enabled by a proprietary Dual-Lock Taq DNA hot-start mechanism.
  • TaqPath master mixes are both Fast and Standard cycling compatible.
  • TaqPath master mixes are optimized for highly multiplexed applications.
I would like to order a TaqMan SNP Genotyping Assay. Does the kit include everything I need to perform my experiment or do I need to purchase reagents separately?

Each predesigned TaqMan SNP Genotyping Assay is delivered in a single tube consisting of two differentially labeled allele-specific TaqMan MGB (minor groove binding) probes and a pair of PCR primers. For more information, please see the following link: https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/real-time-pcr-applications/genetic-variation-analysis-using-real-time/snp-genotyping-with-real-time-pcr.html

Besides the TaqMan SNP Genotyping Assay, you would need to purchase a Genotyping Master Mix and nuclease-free water. A list of compatible master mixes can be found in the following link: https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0009593_TaqManSNP_UG.pdf.