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Applied Biosystems™ TaqMan™ Reagents Starter Kit

Catalog No. 4352405
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Catalog No. 4352405 Supplier Applied Biosystems™ Supplier No. 4352405
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Description

Includes

TaqMan™ Universal PCR Master Mix, No AmpErase™ UNG, Eukaryotic 18S rRNA TaqMan™ Assay (with two unlabeled PCR primers and a labeled TaqMan™ probe), plus a sample of human Raji cDNA

TaqMan™ PCR Starter Kit is designed to detect and quantify Eukaryotic 18S rRNA sequence in human Raji cDNA using the TaqMan™ 5' nuclease assay with the TaqMan™ PCR Master Mix.

Gene-specific, ready to use TaqMan™ Gene Expression Assays and Universal Master Mix provide you with a fast, simple way for measuring changes in gene expression.

  • Simple procedure gives you quick results
  • Hot-Start DNA Polymerase enzyme system minimizes nonspecific product amplification for reliable results
  • Used With: 7500 Real-Time PCR System with Dell™ Notebook, 7500 Real-Time PCR System with Dell™ Tower, 7900HT Fast Real-Time PCR System with 384-well Block Module, 7900HT Fast Real-Time PCR System with 384-well Block Module and Automation Accessory, 7900HT Fast Real-Time PCR System with Standard 96-well Block Module, 7900HT System Automation Accessory Upgrade, 7900HT System Standard 384-well Block Upgrade Kit, 7900HT System Standard 96-well Block Upgrade Kit, ABI PRISM™ 7000 Sequence Detection System (Notebook)

Ready-to-Use Gene Expression Solution:
  • The TaqMan™ Reagents Starter Kit contains a sample of TaqMan™ Universal PCR Master Mix, and TaqMan™ Gene-Expression Assay (Eukaryotic 18S rRNA), plus a sample of human Raji cDNA - everything you need for 18S gene-expression analysis
  • Simply combine these reagents according to the instructions to generate reproducible and quantitative gene-expression data

A Complete, Integrated Gene-Expression System:
  • Eliminate time-consuming master mix formulation and assay optimization with the TaqMan™ Gene-Expression Assays and the TaqMan™ Universal PCR Master Mix, by using universal cycling conditions and pre-optimized reagents

Order Info

Shipping Conditions: Dry Ice

Specifications

Content And Storage This kit contains TaqMan™ Universal PCR Master Mix, No AmpErase™ UNG, Eukaryotic 18S rRNA TaqMan™ Assay (with two unlabeled PCR primers and a labeled TaqMan™ probe), plus a sample of human Raji cDNA.

Store the PCR mix at 2° to 8°C.
Store the rest of the kit contents at -15° to -25°C.
Detection Method Primer-probe
PCR Method qPCR
Polymerase AmpliTaq Gold DNA Polymerase
Reaction Speed Standard
For Use With (Equipment) 7000 System, 7500 System, AB 7900 HT 384-Well System
Passive Reference Dye ROX (Pre-mixed)
Product Line TaqMan
Product Type PCR Reagents
Quantity 1 kit
Shipping Condition Dry Ice
Target 18S rRNA
Target Specificity Target-Specific
For Use With (Application) Gene Expression
Hot Start Built-In Hot Start
Sample Type DNA (Genomic), cDNA
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For Research Use Only. Not for use in diagnostic procedures.