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  7. Applied Biosystems™ TaqMan™ Gene Expression Master Mix

Applied Biosystems™ TaqMan™ Gene Expression Master Mix

Catalog No. 4370048
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Catalog No. 4370048 Supplier Applied Biosystems™ Supplier No. 4370048
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Description

Requires

Template and primers

Precise and reliable real-time qPCR quantification over 9 logs of linear dynamic range with Applied Biosystems™ TaqMan™ Gene Expression Master Mix.

TaqMan™ Gene Expression Master Mix has been validated with TaqMan™ Gene Expression Assays and Applied Biosystems™ real-time systems, providing precise quantification for a variety of gene expression-based real-time qPCR applications, including rare transcript detection, gene expression analysis, pathogen detection and viral load quantification,co-amplification of two targets, as well as specific detection of gene family members

TaqMan™ Gene Expression Master Mix contains AmpliTaq Gold™ DNA Polymerase, UP (Ultra Pure) for hot start activation and improved detection of bacterial targets, a blend of dNTPs with dTTP/dUTP and Uracil-DNA Glycosylase (UDG) to minimize carry-over PCR contamination, and a passive internal reference based on proprietary ROX™ dye for superb precision on Applied Biosystems™ real-time PCR instruments.

  • Provides precise and reliable real-time qPCR quantification over nine logs of linear dynamic range
  • TaqMan Gene Expression Master Mix is an optimized 2X mix that contains all of the components, excluding the template and primers, for sensitive detection down to one copy of target
  • Extended benchtop stability provides consistent results for automated liquid-handling systems
  • This makes it the choice for high-throughput sample processing overnight or over the weekend
  • Validated with TaqMan gene expression assays and Applied Biosystems real-time systems, providing precise quantification for a variety of gene expression–based real-time qPCR applications
  • Applications include rare transcript detection, gene expression analysis, pathogen detection and viral load quantification, co-amplification of two targets, as well as specific detection of gene family members
  • Store at 2°C to 8°C

TaqMan Gene Expression Master Mix contains:

  • AmpliTaq Gold DNA Polymerase, UP (Ultra Pure), for hot start activation and improved detection of bacterial targets
  • A blend of dNTPs with dTTP/dUTP and uracil-DNA glycosylase (UDG) to minimize carry-over PCR contamination
  • A passive internal reference based on proprietary ROX dye for superb precision on Applied Biosystems real-time PCR instruments

Order Info

Shipping Condition: Wet ice

Specifications

Content And Storage 1 tube (1 mL) 2X TaqMan™ Gene Expression Master Mix sufficient for 40 reactions. Store at 2°C to 8°C.


TaqMan™ Gene Expression Master Mix contains AmpliTaq Gold™ DNA Polymerase (Ultra Pure), Uracil-DNA glycosylase, dNTPs (with dUTP), ROX™ Passive Reference, and optimized buffer components.
Detection Method Primer-probe
Form Liquid
PCR Method qPCR
Polymerase AmpliTaq Gold DNA Polymerase
Reaction Speed Standard
For Use With (Equipment) 7000 System, 7300 System, 7500 System, 7900HT System, Applied Biosystems StepOnePlus™ Fast Real-Time PCR SystemStepOne™, Standard Mode, StepOnePlus™, Standard Mode
Passive Reference Dye ROX (Pre-mixed)
Product Line TaqMan
Purity or Quality Grade UP (Ultra Pure)
Quantity 1 x 1 mL
For Use With (Application) Gene Expression
No. of Reactions 40 Reactions
Sample Type DNA (Genomic), cDNA
Volume 1 x 1 mL
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For Research Use Only. Not for use in diagnostic procedures.