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  7. Applied Biosystems™ SYBR™ Select Master Mix for CFX

Applied Biosystems™ SYBR™ Select Master Mix for CFX

Catalog No. 4472954
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Catalog No. 4472954 Supplier Applied Biosystems™ Supplier No. 4472954
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Description

Includes

2X mix contains SYBR GreenER dye, AmpliTaq DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, and optimized buffer components.

A cost-effective solution for real-time PCR applications.

  • Specific - minimize primer-dimer and non-specific amplification
  • Reproducible and sensitive - consistent amplification across a wide dynamic range
  • Bright - contains SYBR™ GreenER™ dye for maximum brightness
  • Carry-over contamination control - contains heat-labile UDG
  • For use with the Bio-Rad CFX96™ Touch or CFX384™ Touch systems
  • Standard and fast cycling parameters

Formulated for Maximum Specificity:
  • SYBR™ Select Master Mix for CFX contains all the components needed for your real-time PCR reactions, except the template and primers, in a convenient 2X concentration premix
  • The master mix includes AmpliTaq™ DNA Polymerase, UP, a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity

Obtain Reproducible Results Across a Wide Dynamic Range:
  • SYBR™ Select Master Mix for CFX is specially formulated to provide robust results from 100ng to 0.1pg cDNA per reaction and can reliably detect a single copy of genomic DNA.
    • Contains SYBR™ GreenER™ Dye and Heat-labile UDG:
    • SYBR™ GreenER™ dye is less inhibitory to PCR than SYBR™ Green I dye, resulting in brighter signals
    • Heat-labile uracil-DNA glycosylase (UDG) is included for worry-free carryover contamination control

Order Info

Shipping Conditions: Wet Ice

Specifications

Concentration 2X
Content And Storage The 2x mix contains SYBR™ GreenER™ dye, AmpliTaq™ DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, and optimized buffer components. Contains 10 X 5 mL bottles, sufficient for 5000 reactions at 20 μL. Store at 2-8™C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Detection Method SYBR
Form Liquid
Format Vial
GC-Rich PCR Performance High
PCR Method qPCR
Polymerase AmpliTaq DNA Polymerase
Reaction Speed Fast or Standard
For Use With (Equipment) AB 7900HT, Standard Mode, AB StepOne™, Fast Mode, AB 7500, Standard Mode, AB 7500, Fast Mode, Bio-Rad CFX96 Touch™, AB StepOnePlus™, Fast Mode, AB 7900HT, Fast Mode, AB StepOne™, Standard Mode, Bio-Rad CFX Connect™, AB 7900 HT 384-Well System, Bio-Rad CFX384 Touch™, AB StepOnePlus™, Standard Mode
Label or Dye SYBR GreenER
Passive Reference Dye ROX (Pre-mixed)
Product Line SYBR
Product Type Real Time PCR SYBR Master Mix
Purity or Quality Grade UP (Ultra Pure)
Quantity 10 x 5 mL
Sufficient For 5000 Reactions of 20 μL
For Use With (Application) Gene Expression
Hot Start Built-In Hot Start
No. of Reactions 5000 Reactions
Sample Type DNA (Genomic), cDNA
Volume 10 x 5 mL
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Frequently Asked Questions (FAQs)

How does the SYBR GreenER Dye Chemistry work?

The SYBR GreenER dye binds to double stranded DNA formed during PCR. During PCR, the polymerase amplifies the target sequence which creates the amplicon. As PCR continues, the dye binds to each new copy of double-stranded DNA, leading to more amplicons being created. The result is an increase in fluorescence intensity proportional to the amount of double-stranded PCR product produced.

For Research Use Only. Not for use in diagnostic procedures.