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  7. Applied Biosystems™ SYBR™ Select Master Mix for CFX

Applied Biosystems™ SYBR™ Select Master Mix for CFX

Numéro de catalogue. 4472954
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4472954 10 x 5 mL
4472937 1 x 1 mL
4472942 1 x 5 mL
4472952 2 x 5 mL
4472953 5 x 5 mL
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Numéro de catalogue. 4472954 Fournisseur Applied Biosystems™ Code fournisseur 4472954
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Description

Comprend

2X mix contains SYBR GreenER dye, AmpliTaq DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, and optimized buffer components.

A cost-effective solution for real-time PCR applications.

  • Specific - minimize primer-dimer and non-specific amplification
  • Reproducible and sensitive - consistent amplification across a wide dynamic range
  • Bright - contains SYBR™ GreenER™ dye for maximum brightness
  • Carry-over contamination control - contains heat-labile UDG
  • For use with the Bio-Rad CFX96™ Touch or CFX384™ Touch systems
  • Standard and fast cycling parameters

Formulated for Maximum Specificity:
  • SYBR™ Select Master Mix for CFX contains all the components needed for your real-time PCR reactions, except the template and primers, in a convenient 2X concentration premix
  • The master mix includes AmpliTaq™ DNA Polymerase, UP, a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity

Obtain Reproducible Results Across a Wide Dynamic Range:
  • SYBR™ Select Master Mix for CFX is specially formulated to provide robust results from 100ng to 0.1pg cDNA per reaction and can reliably detect a single copy of genomic DNA.
    • Contains SYBR™ GreenER™ Dye and Heat-labile UDG:
    • SYBR™ GreenER™ dye is less inhibitory to PCR than SYBR™ Green I dye, resulting in brighter signals
    • Heat-labile uracil-DNA glycosylase (UDG) is included for worry-free carryover contamination control

Order Info

Shipping Conditions: Wet Ice

Spécifications

Concentration 2X
Content And Storage The 2x mix contains SYBR™ GreenER™ dye, AmpliTaq™ DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, and optimized buffer components. Contains 10 X 5 mL bottles, sufficient for 5000 reactions at 20 μL. Store at 2-8™C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Detection Method SYBR
Form Liquid
Format Vial
GC-Rich PCR Performance High
PCR Method qPCR
Polymerase AmpliTaq DNA Polymerase
Reaction Speed Fast or Standard
For Use With (Equipment) AB 7900HT, Standard Mode, AB StepOne™, Fast Mode, AB 7500, Standard Mode, AB 7500, Fast Mode, Bio-Rad CFX96 Touch™, AB StepOnePlus™, Fast Mode, AB 7900HT, Fast Mode, AB StepOne™, Standard Mode, Bio-Rad CFX Connect™, AB 7900 HT 384-Well System, Bio-Rad CFX384 Touch™, AB StepOnePlus™, Standard Mode
Label or Dye SYBR GreenER
Passive Reference Dye ROX (Pre-mixed)
Product Line SYBR
Product Type Real Time PCR SYBR Master Mix
Purity or Quality Grade UP (Ultra Pure)
Quantity 10 x 5 mL
Sufficient For 5000 Reactions of 20 μL
For Use With (Application) Gene Expression
Hot Start Built-In Hot Start
No. of Reactions 5000 Reactions
Sample Type DNA (Genomic), cDNA
Volume 10 x 5 mL
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For Research Use Only. Not for use in diagnostic procedures.