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Applied Biosystems™ Proteinase K for MagMAX™ DNA Multi-Sample Ultra Kit

Catalog No. A25561
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A25561 4 mL
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Catalog No. A25561 Supplier Applied Biosystems™ Supplier No. A25561
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Description

This is the same proteinase K found in the MagMAX™ DNA Multi-Sample Ultra Kit (Cat. No. A25597, A25598), available separately in case more proteinase K is required for a particular application.

This is the same proteinase K found in the MagMAX™ DNA Multi-Sample Ultra Kit (Cat. No. A25597, A25598), available separately in case more proteinase K is required for a particular application.

Specifications

Content And Storage Store in freezer (-5 to -30°C).
Format Kit
For Use With (Application) DNA Extraction
Product Line MagMAX
Product Type Proteinase K
Quantity 4 mL

Frequently Asked Questions (FAQs)

What is the concentration of Proteinase K enzyme in Proteinase K for MagMAX DNA Multi-Sample Ultra Kit (Cat. No. A25561)?

The Proteinase K is provided at a concentration of 100 mg/mL.
What type of samples is the MagMAX DNA Multi-Sample Ultra Kit (Cat. No. A25597) optimized for?

We have optimized protocols for extracting DNA from buccal swabs, whole blood, blood cards (Whatman FTA), oral rinse, saliva, and urine samples. In addition, the kit can be used to process vaginal microbiota samples (e.g., ThinPrep, Surepath) for gDNA extraction from yeast, bacteria (Gram positive/negative), viruses, and protozoa with the addition of additional enzymatic steps.

In the MagMAX DNA Multi-Sample Ultra Kit protocol, why do blood samples appear cloudy after adding the Multi-Sample DNA Lysis Buffer?

On rare occasions, the sample may turn cloudy after adding the Multi-Sample DNA Lysis Buffer. The sample will clear up when isopropanol is added. This will not have any impact on the yield or quality of the DNA.
Can heparin be used to collect blood samples for the MagMAX DNA Multi-Sample Ultra Kit?

Heparin is not recommended as an anti-coagulant since it can cause inhibition of PCR reactions. Instead, we strongly recommend collecting blood samples in EDTA or sodium citrate.
What is the best way to remove the buccal swabs from the MagMAX DNA Multi-Sample Ultra Kit lysate?

We recommend transferring the lysate to a new MagMAX Express-96 Deep Well Plate (Cat. No. 4388476). This option eliminates contamination risks and saves time. To transfer lysates, set a multi-channel micropipetor to ˜300 µL and transfer one row at a time. Each well should contain 200-250 µL after transfer.
Can buccal swabs that are to be used with the MagMAX DNA Multi-Sample Ultra Kit be shipped after sample collection?

Buccal swabs can be shipped at 25 degrees C or below. Exposure of the swabs to high temperatures (over 30 degrees C) during shipment has been associated with poor performance in the workflow. To prevent degradation, ensure that buccal swabs are completely dry before shipment. Place the swabs in a paper envelope for storage after collection - paper envelopes are permeable and allow the swabs to dry effectively.
What buccal swabs do you recommended using with the MagMAX DNA Multi-Sample Ultra Kit?

We recommend using one of the following types of buccal swabs with foam tips:

- Puritan PurFlock Ultra Flocked Swabs (Fisher Scientific, Cat. No. 22-025-192)
- Puritan HydraFlock Swabs, standard tip (Puritan, Cat. No. 25-3306-H )
- Sterile Foam Tipped Swabs (Puritan, Cat. No. 25-1506 1PF)
- 4N6FLOQSwabs, regular tip (Thermo Fisher Scientific, Cat. No. 4473979)

Note: Please do not use cotton swabs, as they may contain PCR inhibitors.

Why does the MagMAX DNA Multi-Sample Ultra Kit have two DNA elution buffers?

DNA Elution Buffer 1 is at a basic pH to help maximize release of the gDNA from the magnetic beads and has to be neutralized by DNA Elution Buffer 2.
Why is no RNase treatment included in the MagMAX DNA Multi-Sample Ultra Kit workflow?

RNase A treatment is not critical for this workflow. RNA that might be present in the samples is likely to be degraded in the course of the experiment and should not interfere with downstream applications. For those sample types that might potentially have some RNA carryover, we have included the RNase A as part of the Bead mix as a precaution.
Can I combine DNA Elution Buffer 1 and DNA Elution Buffer 2 from the MagMAX DNA Multi-Sample Ultra Kit and just have a single elution step?

Yes, you can combine the two elution buffers prior to eluting the DNA; instead of having the instrument pause, requiring you to add DNA Elution Buffer 2, press ‘start' on the instrument - just be sure to combine them directly before adding to the elution plate and before putting the plate on the KingFisher instrument (you do not want them to sit as a mix for too long). For example, if you wish to elute in 50 µL of buffer, you can mix 25 µL of DNA Elution Buffer 1 and 25 µL of DNA Elution Buffer 2, then add the total 50 µL into a well and run the protocol as you normally would.

For Research Use Only. Not for use in diagnostic procedures.