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Applied Biosystems™ Power SYBR™ Green PCR Master Mix

Offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations.

Supplier: Applied Biosystems™ 4368702

Catalog No. 4368702

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Description

Get superior nucleic acid quantitation with Applied Biosystems™ Power SYBR™ Green Master Mix.

Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient single-tube format
Power SYBR™ Green PCR Master Mix contains all of the components (excluding the template and primers) for superior SYBR™ Green reagent-based real-time PCR
The optimized formulation contains highly purified AmpliTaq Gold™ DNA Polymerase, LD to offer increased sensitivity
Contains a blend of dTTP/dUTP that is compatible with AmpErase™ UNG to minimize carryover contamination
Includes a proprietary version of ROX™ dye, an internal passive reference, to normalize non-PCR–related fluorescence fluctuations
This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error and sample evaporation
Power SYBR™ Green reagent-based PCR chemistry easily replaces the existing SYBR™ Green PCR Master Mix in Applied Biosystems™ protocols using the same setup and thermal cycling conditions
Store at -20°C

Specifications

Content And Storage	The 2X mix contains SYBR™ Green 1 Dye, AmpliTaq Gold™ DNA Polymerase LD, dNTPs with dUTP/dTTP blend, Passive Reference 1, and optimized buffer components. Contains 1 × 50 mL bottle, sufficient for 1000 reactions of 50 μL each. Store at -20°C. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Format	Bottle
PCR Method	qPCR
Reaction Speed	Standard
Label or Dye	SYBR Green
Product Line	SYBR™
Purity or Quality Grade	LD (Low DNA)
Sufficient For	1000 Reactions of 50 μL Each
Concentration	2X
Sample Type	DNA (Genomic), cDNA

Detection Method	SYBR
GC-Rich PCR Performance	High
Polymerase	AmpliTaq Gold DNA Polymerase, AmpliTaq Gold LD
For Use With (Equipment)	7300 System, 7500 System, 7700 System, 7900HT Fast System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Passive Reference Dye	ROX (Pre-mixed)
Product Type	Real Time PCR SYBR Master Mix
Quantity	5 x 5 mL
For Use With (Application)	Gene Expression
No. of Reactions	1000 Reactions
Volume	5 x 5 mL

Product Suggestions Documents

Documents

Quick Reference Card: Power SYBR Green PCR Master Mix on CFX96 Version: 10 Oct 2008 File size: 161.2 kB

Protocol: Power SYBR Green PCR Master Mix and RT-PCR Version: 4367218 Revision E File size: 1 MB

Quick Reference Card: Power SYBR Green PCR Master Mix on iQ5 Version: 10 Oct 2008 File size: 160.2 kB

Quick Reference Card: Power SYBR Green PCR Master Mix LC 480 Version: 10 Oct 2008 File size: 161.4 kB

Brochures & Specifications: Power SYBR® Green PCR Master Mix (English ) File size: 213.6 kB

Brochures & Specifications: Applied Biosystems SYBR® Green Master Mixes on the Roche LightCycler® 480 Real-Time PCR System (English ) File size: 896.5 kB

Brochures & Specifications: Applied Biosystems SYBR® Green Master Mixes on the Bio-Rad CFX96 Real-Time PCR Detection System (English ) File size: 849.5 kB

Brochures & Specifications: Applied Biosystems SYBR® Green Master Mixes on the Bio-Rad iQ5 Real-Time PCR Detection System (English ) File size: 1.1 MB

Quick Reference Card: Power SYBR® Green PCR Master Mix: Performing Real-Time PCR Assays File size: 61.7 kB

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For Research Use Only. Not for use in diagnostic procedures.