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  7. Applied Biosystems™ GeneAmp™ 10X PCR Buffer II & MgCl2

Applied Biosystems™ GeneAmp™ 10X PCR Buffer II & MgCl2

Catalog No. N8080010
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1 x 1.5 mL
6 x 1.5 mL
2 x 75 mL
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N8080010 1 x 1.5 mL
4379878 2 x 75 mL
N8080130 6 x 1.5 mL
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Catalog No. N8080010 Supplier Applied Biosystems™ Supplier No. N8080010
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Description

Includes

2 x 75mL each of GeneAmp™ 10X PCR Buffer II and MgCl2 sufficient for 100 PCR amplifications of 100μL each.

Applied Biosystems GeneAmp 10X PCR Buffer II is optimized for use with AmpliTaq DNA Polymerase, resulting in robust PCR amplification.

Applied Biosystems™ GeneAmp™ 10X PCR Buffer II is optimized for use with AmpliTaq™ DNA Polymerase, resulting in robust PCR amplification. The provided MgCl2 solution offers versatility for optimizing PCR.

  • Optimized for use with AmpliTaq™ DNA Polymerase, offering maximum PCR performance
  • Available in several convenient sizes
  • Separate MgCl2 solution offers versatility

GeneAmp™ 10X PCR Buffer II, when diluted 1:10, provides the optimal pH and ionic strength for PCR amplification. GeneAmp™ 10X PCR Buffer II is magnesium ion free. A separate MgCl2 solution is provided. When used together, GeneAmp™ 10X PCR Buffer II and MgCl2 solution offer more versatility in optimizing the magnesium ion concentration to achieve optimal PCR amplification with any specific set of primers and DNA template.

Note: See user's manual or package insert for limited label license and trademark information.

Order Info

Shipping Condition: Wet ice

Specifications

Concentration 10X
Content And Storage • GeneAmp 10X PCR Buffer II, 1 x 1.5 mL
• MgCl2, 1 x 1.5 mL

Store at -15°C to -25°C.
Polymerase AmpliTaq DNA Polymerase
Product Type PCR Buffer
Quantity 1 x 1.5 mL
Shipping Condition Dry Ice
For Use With (Application) Standard PCR
Fidelity (vs. Taq) 1X
No. of Reactions 100 Reactions
Reaction Format Kit
Volume 1.5 mL
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Frequently Asked Questions (FAQs)

Can the RT buffer for TaqMan Reverse Transcription Reagents kit (Cat. No. N8080234) be purchased separately?

Yes, it is the same as the GeneAmp 10X PCR Buffer II & MgCl2 (Cat. No. N8080010).

What are the differences between GeneAmp 10X PCR Buffer I, PCR Buffer II and PCR Gold Buffer, and what are the formulations of each buffer?

The ionic strength and the pH of GeneAmp 10X PCR Gold Buffer have been carefully optimized for use with the AmpliTaq Gold enzyme. GeneAmp 10X PCR Buffer I contains Mg; Geneamp 10X PCR Buffer II and Geneamp 10X PCR Gold Buffer do not have Mg in them, but come with a separate vial of 25 mM MgCl2 so that you can titrate the Mg in your PCR reaction.

Buffer formulations:
Buffer I (500 mM KCl, 100 mM Tris-HCl, pH 8.3, 15 mM MgCl2, 0.01% (w/v) gelatin)
Buffer II (500 mM KCl, 100 mM Tris-HCl, pH 8.3), with separate tube of 25 mM MgCl2
Gold Buffer (500 mM KCl, 150 mM Tris-HCl pH 8.0), with separate tube of 25 mM MgCl2

For Research Use Only. Not for use in diagnostic procedures.