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  7. Applied Biosystems™ TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG

Applied Biosystems™ TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG

Catalog No. 4367846
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Catalog No. No. of Reactions
4367846 5000 Reactions
4366072 500 Reactions
4352042 250 Reactions
4366073 1250 Reactions
4364103 2500 Reactions
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Catalog No. 4367846 Supplier Applied Biosystems™ Supplier No. 4367846

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

Requires

Template and primers

TaqMan™ Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR.

  • Results in 40 minutes for 40 cycles of PCR in a 20 μL reaction volume when using the Applied Biosystems 7900HT and Fast 7500 Fast real-time PCR systems
  • The optimized formulation contains AmpliTaq Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and allowing room temperature reaction setup
  • A proprietary ROX dye serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations, for superb precision on Applied Biosystems real-time PCR instruments
  • Contains everything needed for TaqMan-based qPCR amplification and detection in a single-tube format
  • TaqMan Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR
  • Compatible with TaqMan Gene Expression assays
  • Store at 2°C to 8°C

Order Info

Guaranteed minimum shelf life is 60 days.

Specifications

Concentration 2X
Content And Storage TaqMan™ Fast Universal PCR Master Mix, No AmpErase™ UNG Kit contains one 50 mL tube sufficient for 5000 20-μl PCR reactions. Store at 2-8°C

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Detection Method Primer-probe
Format Tube
GC-Rich PCR Performance High
PCR Method qPCR
Polymerase AmpliTaq DNA Polymerase
Reaction Speed Fast
For Use With (Equipment) 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Passive Reference Dye ROX (Pre-mixed)
Product Line AmpErase, TaqMan
Product Type Fast Universal PCR Master Mix
Quantity 5,000 reactions
Shipping Condition Wet Ice
Sufficient For 5000 Reactions
For Use With (Application) Gene Expression, Genotyping, miRNA analysis
Fidelity (vs. Taq) 2 X
No. of Reactions 5000 Reactions
Sample Type DNA (Genomic), cDNA
Volume 20 μL
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Frequently Asked Questions (FAQs)

Can I set up and store the TaqMan Fast Universal PCR Master Mix at room temperature if the instrument is in use?

For optimal results, we recommend to run the reaction plate right after setting up the reaction. If a reaction plate cannot be run within 2 hours of completing the reaction setup, then freeze/refrigerate the reaction plate until it can be loaded and run on the fast PCR instrument platform.
Is the TaqMan Fast Universal PCR Master Mix offered with AmpErase UNG?

The TaqMan Fast Universal PCR Master Mix is provided without any UNG and you should add in AmpEraseUNG to reactions separately, with the addition of an initial 2 minute cycling step at 50º C. If you prefer to have a master mix with the UNG pre-added, please use TaqMan Fast Advanced Master Mix.

What are the storage conditions and the shelf life of the TaqMan Fast Universal PCR Master Mix?

We recommend a storage temperature of 4C and the shelf life is 9 months from the manufacturing date.
Can I use TaqMan Fast Universal PCR Master Mix for both one-step and two-step RT-PCR?

TaqMan Fast Universal PCR Master Mix is recommended for two-step RT-PCR, but for one-step reactions, we recommend using TaqMan Fast Virus 1-Step Master Mix as the preferred reagent.
What component in the TaqMan Fast Universal PCR Master Mix (P/N 4352042) allows rapid amplification results?

The hot-start DNA polymerase enzyme system in the TaqMan Fast Universal PCR Master Mix does not require an activation step, and can be activated with significantly shorter hold times for the thermal cycling stages of PCR, thereby minimizing PCR run time. For more information on using this reagent, please consult the TaqMan Fast Universal PCR Master Mix (2X) protocol.
What is the lowest copy number that can be used with the TaqMan Fast Universal PCR Master Mix (P/N 4352042)?

The fast master mix has demonstrated detection down to 10 copies of the RNase P gene with genomic DNA. Of course, detection limits may vary depending on factors such as assay design and sample preparation.

For Research Use Only. Not for use in diagnostic procedures.