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  7. Applied Biosystems™ TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG

Applied Biosystems™ TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG

Catalog No. 4364103
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No. of Reactions:
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Catalog No. No. of Reactions
4364103 2500 Reactions
4366072 500 Reactions
4367846 5000 Reactions
4352042 250 Reactions
4366073 1250 Reactions
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Catalog No. 4364103 Supplier Applied Biosystems™ Supplier No. 4364103
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Description

Requires

Template and primers

TaqMan™ Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR.

  • Results in 40 minutes for 40 cycles of PCR in a 20 μL reaction volume when using the Applied Biosystems 7900HT and Fast 7500 Fast real-time PCR systems
  • The optimized formulation contains AmpliTaq Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and allowing room temperature reaction setup
  • A proprietary ROX dye serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations, for superb precision on Applied Biosystems real-time PCR instruments
  • Contains everything needed for TaqMan-based qPCR amplification and detection in a single-tube format
  • TaqMan Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR
  • Compatible with TaqMan Gene Expression assays
  • Store at 2°C to 8°C

Order Info

Guaranteed minimum shelf life is 60 days.

Specifications

Concentration 2X
Content And Storage TaqMan™ Fast Universal PCR Master Mix, No AmpErase™ UNG Kit contains 10 packs of 2 tubes (1.25 ml each), sufficient for 2500 PCR reactions (20 μl each).

Store at 2°C to 8°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Detection Method Primer-probe
Format Tube
GC-Rich PCR Performance High
PCR Method qPCR
Polymerase AmpliTaq DNA Polymerase
Reaction Speed Fast
For Use With (Equipment) 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Passive Reference Dye ROX (Pre-mixed)
Product Line AmpErase, TaqMan
Product Type Fast Universal PCR Master Mix
Quantity 2,500 reactions
Sufficient For 2500 Reactions
For Use With (Application) Gene Expression, Genotyping, miRNA analysis
Fidelity (vs. Taq) 2 X
No. of Reactions 2500 Reactions
Sample Type DNA (Genomic), cDNA
Volume 20 μL
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For Research Use Only. Not for use in diagnostic procedures.