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Molecular Probes™ APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU

Catalog No. 501137345
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501137345 60 Assays
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Catalog No. 501137345 Supplier Molecular Probes™ Supplier No. A23210
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Description

APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU

In collaboration with Phoenix Flow Systems, we offer the APO™-BrdU TUNEL Assay Kit which provides all the materials necessary to label and detect the DNA strand breaks of apoptotic cells. When DNA strands are cleaved or nicked by nucleases, a large number of 3´hydroxyl ends are exposed. In the APO-BrdU assay, these ends are labeled with BrdUTP and terminal deoxynucleotidyl transferase (TdT) using the TUNEL technique. Once incorporated ino the DNA, BrdU is detected using a bright and photostable green-fluorescent Alexa Fluor™ 488 dye-labeled anti-BrdU antibody.

Specifications

Color Green
Content And Storage Contains positive control cells (5 mL) and negative control cells (5 mL) of a fixed human lymphoma cell line, 1 vial of TdT (45 μL), 1 vial of BrdUTP (480 μL), 1 vial of anti-BrdU Ms mAb Alexa Fluor™ 488 conjugate (350 μL), 1 bottle of PI/Rnase A staining buffer (30 mL), 1 vial of reaction buffer (0.6 mL), 1 bottle of wash buffer (120 mL) and 1 bottle of rinse buffer (120 mL).

Store as directed.
Detection Method Fluorescence
Format Tube
Excitation/Emission 490/525
Flow Cytometer Laser Lines 488
For Use With (Application) Flow Cytometry
For Use With (Equipment) Fluorescence Microscope, Flow Cytometer
Label or Dye Alexa Fluor™ 488
Product Type TUNEL Assay Kit
Product Line APO-BrdU
Quantity 60 Assays
Species Mouse
Target BrdU
Label Type Alexa Fluor Dyes
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For Research Use Only. Not for use in diagnostic procedures.