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Molecular Probes™ APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU
APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU
Supplier: Molecular Probes™ A23210
Description
In collaboration with Phoenix Flow Systems, we offer the APO™-BrdU TUNEL Assay Kit which provides all the materials necessary to label and detect the DNA strand breaks of apoptotic cells. When DNA strands are cleaved or nicked by nucleases, a large number of 3´hydroxyl ends are exposed. In the APO-BrdU assay, these ends are labeled with BrdUTP and terminal deoxynucleotidyl transferase (TdT) using the TUNEL technique. Once incorporated ino the DNA, BrdU is detected using a bright and photostable green-fluorescent Alexa Fluor™ 488 dye-labeled anti-BrdU antibody.Specifications
| Green | |
| Fluorescence | |
| 490/525 | |
| Flow Cytometry | |
| Alexa Fluor™ 488 | |
| APO-BrdU™ | |
| Mouse | |
| Alexa Fluor Dyes |
| Contains positive control cells (5 mL) and negative control cells (5 mL) of a fixed human lymphoma cell line, 1 vial of TdT (45 μL), 1 vial of BrdUTP (480 μL), 1 vial of anti-BrdU Ms mAb Alexa Fluor™ 488 conjugate (350 μL), 1 bottle of PI/Rnase A staining buffer (30 mL), 1 vial of reaction buffer (0.6 mL), 1 bottle of wash buffer (120 mL) and 1 bottle of rinse buffer (120 mL). Store as directed. |
|
| Tube | |
| 488 | |
| Fluorescence Microscope, Flow Cytometer | |
| TUNEL Assay Kit | |
| 60 Assays | |
| BrdU |
For Research Use Only. Not for use in diagnostic procedures.