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Invitrogen™ Anza™ DNA End Repair Kit

Catalog No. IVGN2504
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20 reactions
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IVGN2504 20 reactions
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Catalog No. IVGN2504 Supplier Invitrogen™ Supplier No. IVGN2504
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Description

Used to convert DNA with cohesive ends to blunt-ended 5'-phosphorylated DNA for use in blunt-end ligation reactions

The Invitrogen™ Anza™ DNA End Repair Kit is used to convert DNA with cohesive ends to blunt-ended 5’-phosphorylated DNA for use in blunt-end ligation reactions. The included pre-mixed Anza™ DNA End Repair Mix contains T4 DNA polymerase, Klenow Fragment, and T4 PNK (polynucleotide kinase) to minimize pipetting, while the Anza™ 10X End Repair Buffer contains ATP and dNTPs to facilitate the activity of the enzyme mix.

Benefits:
  • Convenient—pre-mixed enzyme mixture for superior convenience and reduction of pipetting
  • Efficient—simultaneous blunting and phosphorylation
  • Compatible—use directly in Anza restriction enzyme digestion reaction mixture following heat inactivation
  • Fast—end repair complete in 15 minutes

The Anza DNA End Repair Kit is part of the Anza™ Restriction Enzyme Cloning System, unifying the traditional cloning processes. Other Anza DNA Modifying Enzymes include the Anza T4 DNA Ligase Master Mix, Anza T4 PNK Kit, and Anza DNA Blunt End Kit.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Content And Storage 100 μL Anza DNA End Repair Mix
200 μL Anza 10X End Repair Buffer

Store at -5 to -30°C.
Shipping Condition Approved for shipment on Wet or Dry Ice
Enzyme T4 Polynucleotide Kinase, T4 DNA polymerase, Klenow Fragment
Compatible Buffer Anza™ 10X End Repair Buffer
Quantity 20 reactions
Product Type DNA End Repair Kit
Product Line Anza

For Research Use Only. Not for use in diagnostic procedures.