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Thermo Scientific™ AminoLink™ Coupling Resin

Catalog No. PI20381
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PI20381 10 mL
PI20382 50 mL
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Catalog No. PI20381 Supplier Thermo Scientific™ Supplier No. LSG20381

Description

Covalently immobilize proteins and antibodies by lysine residues or other primary amines (-NH2) via reductive amination with these aldehyde-activated agarose beads.

Thermo Scientific™ AminoLink Coupling Resin is crosslinked 4% beaded agarose that has been activated with aldehyde groups to enable covalent immobilization of antibodies and other proteins through primary amines.

AminoLink Coupling Resin is crosslinked 4% beaded agarose that has been activated with aldehyde groups. Proteins and other molecules with primary amines can be covalently attached (immobilized) to the resin to make chromatography columns for use in affinity purification. The aldehyde groups form stable secondary amine bonds with primary amines such as exist in the side chain of lysine (K) residues, which are generally abundant and readily accessible in proteins. Once a protein is immobilized, the prepared affinity resin can be used for a variety of batch and column affinity purification methods involving binding interactions with the immobilized protein. The resin and linkage are stable in most binding and elution conditions typically used in affinity chromatography, enabling prepared resin to be used for multiple rounds of affinity purification procedures.

Highlights:

  • AminoLink Coupling Resin – aldehyde-activated crosslinked 4% beaded agarose

  • Ideal for antibodies and other proteins – immobilize molecules via primary amines (-NH2)

  • Flexible coupling conditions – efficient (>85%) coupling over a wide range of pH (4 to 10) and buffer conditions (PBS or other non-amine buffer with or without organic solvent

  • Stable, permanent immobilization – coupling reaction results in stable, leak-resistant secondary amine bond between resin and ligand

  • Better than immobilization to CNBr-activated agarose – bond is more stable and uncharged, resulting in less nonspecific binding in affinity purification procedures

  • Versatile and reusable – prepared affinity resin is adaptable to column and batch affinity techniques and the resin is reusable for typical applications based on protein binding interactions

Includes:

Kit contains pre-packed AminoLink Columns, coupling buffer, quenching buffer, wash solution, sodium cyanoborohydride solution, and column accessories

Specifications

Content And Storage Store at 4°C.
Product Line AminoLink
Quantity 10 mL
Target Antibody, Proteins through Primary Amine
Type Resin
Stationary Phase Aldehyde
Format Bottle
Column Type Affinity, Agarose Resin

Frequently Asked Questions (FAQs)

Can you provide the shelf-life for AminoLink Coupling Resin?

AminoLink Coupling Resin is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.
Does sodium azide interfere with AminoLink Support coupling?

No, the gel is stored in sodium azide with no loss in activity. Azide is not a primary amine and is rather unreactive.
What is the difference between AminoLink Coupling Resin and NHS-Activated Agarose?

Both react with primary amines, but AminoLink Coupling Resin is a two-step conjugation. First a Schiff base is formed between the amine and the aldehyde on the resin, which is then reduced to a stable secondary amine with sodium cyanoborohydride, whereas NHS-Activated Agarose utilizes an NHS ester to form an amide bond in a one-step conjugation.
What is the difference between AminoLink and AminoLink Plus supports?

AminoLink Plus Coupling Resin is activated at a higher level and has higher flow rates than the original AminoLink Coupling Resin, resulting in higher capacity and faster purification. AminoLink Supports can be used to immobilize any molecule with a primary amine.
How do your AminoLink Coupling Resin and AminoLink Plus Coupling Resin work?

AminoLink and AminoLink Plus Supports are activated with aldehyde groups which will react with primary amines to form Schiff bases, which are reduced to stable, non-reversible secondary amines. Coupling efficiency often exceeds 85% with this support.

For Research Use Only. Not for use in diagnostic procedures.