Invitrogen™ ULTRAhyb™ Ultrasensitive Hybridization Buffer

Proprietary blend of hybridization accelerators and blocking agents that greatly enhance the levels of hybridization

Supplier: Invitrogen™ AM8670

Numéro de catalogue. AM8670

Quantité

Description

Signals that once took days to visualize are now apparent in hours
It is supplied in four bottles containing 125mL each
Increase the sensitivity of blot hybridization experiments
Increase random-primed DNA probe sensitivity up to 100x; RNA probe sensitivity 20x
Decrease hybridization time to 2 hours
Using standard hybridization buffers, only 1 to 5% of target molecules on a blot hybridizes to probe, making blots a relatively insensitive method for nucleic acid analysis (Vernier et al. (1996) Anal Biochem 235: 11-19); With ULTRAhyb Ultrasensitive Hybridization Buffer, the hybridization reaction approaches completion, so that as few as 10,000 molecules can be detected
Compatible with RNA and DNA probes labeled isotopically and nonisotopically in northerns, Southerns, and dot/slot blots
Contains 50% formamide and is compatible with positively charged membranes
Requires 10-100x less nonisotopic DNA probe than standard hybridization buffers, making it possible to perform many more hybridizations with a single labeling reaction

Applications:

DNA and RNA Purification and Analysis, Northern Blotting, Nucleic Acid Gel Electrophoresis and Blotting

Spécifications

Content And Storage	Store at 4°C.
Product Type	Ultrasensitive Hybridization Buffer
Shipping Condition	Room Temperature

Buffer	Hybridization Buffer
Quantity	125 mL

missing translation for 'recommendations'

For Research Use Only. Not for use in diagnostic procedures.

Description