Invitrogen™ ULTRAhyb™ Ultrasensitive Hybridization Buffer

• Signals that once took days to visualize are now apparent in hours
• It is supplied in four bottles containing 125mL each
• Increase the sensitivity of blot hybridization experiments
• Increase random-primed DNA probe sensitivity up to 100x; RNA probe sensitivity 20x
• Decrease hybridization time to 2 hours
• Using standard hybridization buffers, only 1 to 5% of target molecules on a blot hybridizes to probe, making blots a relatively insensitive method for nucleic acid analysis (Vernier et al. (1996) Anal Biochem 235: 11-19); With ULTRAhyb Ultrasensitive Hybridization Buffer, the hybridization reaction approaches completion, so that as few as 10,000 molecules can be detected
• Compatible with RNA and DNA probes labeled isotopically and nonisotopically in northerns, Southerns, and dot/slot blots
• Contains 50% formamide and is compatible with positively charged membranes
• Requires 10-100x less nonisotopic DNA probe than standard hybridization buffers, making it possible to perform many more hybridizations with a single labeling reaction

Applications:

DNA and RNA Purification and Analysis, Northern Blotting, Nucleic Acid Gel Electrophoresis and Blotting