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Invitrogen™ TaqMan™ PreAmp Cells-to-CT™ Kit
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Catalog No. 4387299
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40 reactions
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4387299 40 reactions
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Catalog No. 4387299 Supplier Invitrogen™ Supplier No. 4387299
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Description

Includes

40 sample prep rxns, 40 Dnase rxns, 40 reverse transcription rxns, and 100 20μL PCR rxns for 40 reactions.

The TaqMan PreAmp Cells-to-CT Kit makes it possible to perform gene expression analysis directly from limited or small numbers of cultured cells without RNA purification.

  • Complete, validated solution: Optimized workflow includes cell lysis reagents, DNase, RT reagents, PreAmp reagents, and TAqMan Gene Expression Master Mix
  • Fast, simple and convenient: Prepare samples in 7 minutes; eliminate tedious RNA isolation
  • Extend limited samples: Increase real-time PCR potential by up to 64-fold; study up to 100 genes at once
  • Accurate results: Uniform amplification without introducing bias
  • Robust performance: Linear detection from 10 to 100,000 cells; results equivalent to purified RNA
  • Fewer resources used, less hazardous, less waste

Order Info

Shipping Condition: Dry ice

Specifications

Content And Storage

• 40 x 20 μL sample prep reactions
• 40 x 20 μL Dnase reactions
• 40 x 20 μL reverse transcription reactions
• 100 x 20 μL PCR reactions
Detection Method Primer-probe
Format Kit
GC-Rich PCR Performance Low
PCR Method 2-step RT-qPCR
Polymerase DNA Polymerase
Reaction Speed Standard
For Use With (Equipment) 7000 System, 7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Passive Reference Dye ROX (Pre-mixed)
Product Line Ambion, Cells-to-CT, TaqMan
Product Type Preamp Cells-to-C Kit
Purity or Quality Grade UP (Ultra Pure)
Quantity 40 reactions
Reverse Transcriptase M-MLV
Shipping Condition Dry Ice
Sufficient For 40 Reactions
For Use With (Application) Pre-amplification
No. of Reactions 40 Reactions
Sample Type Cell
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For Research Use Only. Not for use in diagnostic procedures.