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Ambion™ T4 RNA Ligase (cloned) 5U/μL DFS Item


Catalyzes formation of a phosphodiester linkage between a 5'-phosphoryl-terminated nucleic acid donor and a 3'-hydroxyl-terminated nucleic acid acceptor

Manufacturer: life technologies  AM2141

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Catalog No. AM2141

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Description & Specifications

Specifications

Concentration 5U/μL
For Use With (Application) Mapping studies (RACE), 3'-end labeling RNA molecules, and circularizing RNA and DNA molecules
Includes T4 RNA Ligase and 10X Reaction Buffer
Quantity 2500 units
Sample Type RNA, DNA, and oligonucleotides
Storage Requirements Store at -20deg.C

  • Substrates include RNA, DNA, and oligonucleotides. One tube containing 500U (5U/μL) is provided along with 10X Reaction Buffer
  • The enzyme can be used for tagging the 5' ends of mRNA with oligonucleotides for mapping studies (RACE), 3'-end labeling RNA molecules, and circularizing RNA and DNA molecules
  • Unit Definition: One unit catalyzes the formation of 1nmol of [5'-32P]-rA12-18 into a phosphatase-resistant form in 30 minutes at 37°C

DNA & RNA Purification & Analysis, Nucleic Acid Labeling & Oligo Synthesis, Oligo Labeling, RNA Labeling