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Invitrogen™ Ambion™ T4 RNA Ligase, cloned, 5 U/μL DFS Item

Catalyzes formation of a phosphodiester linkage between a 5'-phosphoryl-terminated nucleic acid donor and a 3'-hydroxyl-terminated nucleic acid acceptor

Supplier:  Invitrogen™ AM2141

Catalog No. AM2141



Description

Description

  • Substrates include RNA, DNA, and oligonucleotides. One tube containing 500U (5U/μL) is provided along with 10X Reaction Buffer
  • The enzyme can be used for tagging the 5′ ends of mRNA with oligonucleotides for mapping studies (RACE), 3′-end labeling RNA molecules, and circularizing RNA and DNA molecules
  • Unit Definition: One unit catalyzes the formation of 1nmol of [5′-32P]-rA12-18 into a phosphatase-resistant form in 30 minutes at 37°C

DNA & RNA Purification & Analysis, Nucleic Acid Labeling & Oligo Synthesis, Oligo Labeling, RNA Labeling

Specifications

Specifications

5 U/μL
Tube
10 X
SP6 RNA Polymerase
T4 Rna Ligase
Dry Ice
Solution
T4 RNA Ligase and 10X Reaction Buffer should be stored at –20°C.
Ligase
250
Ambion™
5 μL
RNA
SDS
Documents

Documents

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For Research Use Only. Not for use in diagnostic procedures.