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Invitrogen™ Ambion™ T4 RNA Ligase, cloned, 5 U/μL
Catalyzes formation of a phosphodiester linkage between a 5'-phosphoryl-terminated nucleic acid donor and a 3'-hydroxyl-terminated nucleic acid acceptor
Supplier: Invitrogen™ AM2141
Description
- Substrates include RNA, DNA, and oligonucleotides. One tube containing 500U (5U/μL) is provided along with 10X Reaction Buffer
- The enzyme can be used for tagging the 5′ ends of mRNA with oligonucleotides for mapping studies (RACE), 3′-end labeling RNA molecules, and circularizing RNA and DNA molecules
- Unit Definition: One unit catalyzes the formation of 1nmol of [5′-32P]-rA12-18 into a phosphatase-resistant form in 30 minutes at 37°C
DNA & RNA Purification & Analysis, Nucleic Acid Labeling & Oligo Synthesis, Oligo Labeling, RNA Labeling
Specifications
5 U/μL | |
Tube | |
10 X | |
SP6 RNA Polymerase | |
T4 Rna Ligase | |
Dry Ice | |
Solution |
T4 RNA Ligase and 10X Reaction Buffer should be stored at –20°C. | |
Ligase | |
250 | |
Ambion™ | |
5 μL | |
RNA |
For Research Use Only. Not for use in diagnostic procedures.