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Invitrogen™ Ambion™ T4 RNA Ligase, cloned, 5 U/μL

Catalog No. AM2141
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2,500 units, 5 μL
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Catalog No. Quantity
AM2141 2,500 units, 5 μL
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Catalog No. AM2141 Supplier Invitrogen™ Supplier No. AM2141
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Description

Catalyzes formation of a phosphodiester linkage between a 5'-phosphoryl-terminated nucleic acid donor and a 3'-hydroxyl-terminated nucleic acid acceptor

  • Substrates include RNA, DNA, and oligonucleotides. One tube containing 500U (5U/μL) is provided along with 10X Reaction Buffer
  • The enzyme can be used for tagging the 5′ ends of mRNA with oligonucleotides for mapping studies (RACE), 3′-end labeling RNA molecules, and circularizing RNA and DNA molecules
  • Unit Definition: One unit catalyzes the formation of 1nmol of [5′-32P]-rA12-18 into a phosphatase-resistant form in 30 minutes at 37°C

DNA & RNA Purification & Analysis, Nucleic Acid Labeling & Oligo Synthesis, Oligo Labeling, RNA Labeling

Specifications

Concentration 5 U/μL
Content And Storage T4 RNA Ligase and 10X Reaction Buffer should be stored at –20°C.
Format Tube
Enzyme Ligase
Fidelity (vs. Taq) 10 X
No. of Reactions 250
Polymerase SP6 RNA Polymerase
Product Line Ambion
Product Type T4 Rna Ligase
Quantity 2,500 units, 5 μL
Shipping Condition Dry Ice
Source RNA
Form Solution
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For Research Use Only. Not for use in diagnostic procedures.