RNase I degrades all RNA dinucleotide bonds leaving a 5' hydroxyl and 2', 3' cyclic monophosphate. RNase I will degrade any RNA to mixture of mononucleotides, dinucleotides, and trinucleotides and does not degrade DNA, although it will bind to DNA. It has marked preference for single-stranded RNA over double-stranded RNA, which allows it to work well in ribonuclease protection assays.
- Supplied in one tube of 10,000U (100U/μL)
- Endonuclease-free, exonuclease-free, protease-free
- Rigorously tested for nonspecific contaminating endonuclease, exonuclease, and protease activity
- Unit Definition: One unit is amount of enzyme required to produce 1μg of acid-soluble material from mouse liver RNA in 30 min. at 37°C
DNA & RNA Purification & Analysis, Nuclease Protection Assays, Nucleic Acid Gel Electrophoresis & Blotting
Shipping Conditions: Dry ice
|Store at -20°C|