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  5. Invitrogen™ Ambion™ RNase I, cloned, 100 U/μL

Invitrogen™ Ambion™ RNase I, cloned, 100 U/μL

Catalog No. AM2294
Encompass
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10,000 U
25,000 U
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Catalog No. Quantity
AM2294 10,000 U
AM2295 25,000 U
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Catalog No. AM2294 Supplier Invitrogen™ Supplier No. AM2294

Description

Efficiently cleaves after all four bases of single-stranded RNA, in contrast to RNase A, which only cleaves after C and U residues

RNase I degrades all RNA dinucleotide bonds leaving a 5' hydroxyl and 2', 3' cyclic monophosphate. RNase I will degrade any RNA to mixture of mononucleotides, dinucleotides, and trinucleotides and does not degrade DNA, although it will bind to DNA. It has marked preference for single-stranded RNA over double-stranded RNA, which allows it to work well in ribonuclease protection assays.

  • Supplied in one tube of 10,000U (100U/μL)
  • Endonuclease-free, exonuclease-free, protease-free
  • Rigorously tested for nonspecific contaminating endonuclease, exonuclease, and protease activity
  • Unit Definition: One unit is amount of enzyme required to produce 1μg of acid-soluble material from mouse liver RNA in 30 min. at 37°C

DNA & RNA Purification & Analysis, Nuclease Protection Assays, Nucleic Acid Gel Electrophoresis & Blotting

Order Info

Shipping Conditions: Dry ice

Specifications

Concentration 100 U/μL
Content And Storage -20°C
Shipping Condition Dry Ice
Enzyme RNase
Quantity 10,000 U
Product Type RNase I
Product Line Ambion

For Research Use Only. Not for use in diagnostic procedures.