Previously frozen tissues can be processed like fresh samples
$112.00 - $592.00
RNAlater™-ICE is a novel reagent for transitioning frozen tissue to a state that is easily processed for extraction of high quality RNA. Frozen tissues are simply submerged in RNAlater-ICE and allowed to thaw overnight at -20°C. Once thawed, the tissues can be processed like fresh tissues using standard RNA isolation procedures. No more laborious grinding of frozen tissue to safeguard the RNA. Often, tissues that need to be stored prior to RNA isolation are “snap” or “flash” frozen on dry ice or in liquid nitrogen to preserve RNA integrity. RNA in tissue is stable while frozen at -80°C but thawing the tissue prior to or during its disruption can result in RNA degradation. This is true even if the tissue thaws while in the denaturation solution.
DNA and RNA Purification and Analysis, General RNA Purification Reagents and Accessories, RNA Extraction, Total RNA Isolation, mRNA Isolation