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Invitrogen™ Ambion™ Ribonuclease H, from E. coli (cloned) 10U/μL DFS Item

Endonuclease-free, exonuclease-free, protease-free

$114.45 - $318.15

Specifications

Storage Requirements Store at -20°C
View More Specs

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

AM2293

DFS Item
 
invitrogen™
AM2293
1000 units Each for $318.15

AM2292

DFS Item
 
invitrogen™
AM2292
200 units Each for $114.45
Description & Specifications

Specifications

Storage Requirements Store at -20°C

Ribonuclease H (RNase H) is isolated from an E. coli strain that over-expresses the gene. RNase H specifically degrades RNA in RNA:DNA hybrids to produce 3'-hydroxyl and 5'-phosphate terminated products.

  • Will not degrade DNA or unhybridized RNA
  • Integral part of most RNA amplification and NASBA protocols and can be used to degrade specific RNAs when complementary DNA oligo is hybridized, such as poly(A) tail removal from mRNA hybridized to oligo(dT)
  • Rigorously tested for contaminating nonspecific endonuclease, exonuclease, RNase, and protease activity
  • Supplied in one tube containing 200U (10U/μL
  • Unit Definition: One unit of Ribonuclease H is amount of enzyme required to increase fluorescence 1.5 RFUs per sec. at 37°C using 20pmol of RNaseAlert™ probe coupled to 1000pmol of a complementary oligonucleotide as substrate

PCR & Real-Time PCR, Real Time PCR (qPCR), Reverse Transcription

Shipping Conditions: Dry ice