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  5. Invitrogen™ Ambion™ RNase H, from E. coli, 10 U/μL

Invitrogen™ Ambion™ RNase H, from E. coli, 10 U/μL

Catalog No. AM2293
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1000 U
200 U
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Catalog No. Quantity
AM2293 1000 U
AM2292 200 U
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Catalog No. AM2293 Supplier Invitrogen™ Supplier No. AM2293
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Description

Endonuclease-free, exonuclease-free, protease-free

Ribonuclease H (RNase H) is isolated from an E. coli strain that over-expresses the gene. RNase H specifically degrades RNA in RNA:DNA hybrids to produce 3'-hydroxyl and 5'-phosphate terminated products.

  • Will not degrade DNA or unhybridized RNA
  • Integral part of most RNA amplification and NASBA protocols and can be used to degrade specific RNAs when complementary DNA oligo is hybridized, such as poly(A) tail removal from mRNA hybridized to oligo(dT)
  • Rigorously tested for contaminating nonspecific endonuclease, exonuclease, RNase, and protease activity
  • Supplied in one tube containing 200U (10U/μL
  • Unit Definition: One unit of Ribonuclease H is amount of enzyme required to increase fluorescence 1.5 RFUs per sec. at 37°C using 20pmol of RNaseAlert™ probe coupled to 1000pmol of a complementary oligonucleotide as substrate

PCR & Real-Time PCR, Real Time PCR (qPCR), Reverse Transcription

Order Info

Shipping Conditions: Dry ice

Specifications

Concentration 10 U/μL
Content And Storage -20°C
Shipping Condition Dry Ice
Enzyme RNase
Quantity 1000 U
Product Type RNase H
Product Line Ambion

For Research Use Only. Not for use in diagnostic procedures.