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Invitrogen™ Recombinant Proteinase K Solution (20 mg/mL) 
Recombinant Proteinase K is a nonspecific serine protease that is useful for general digestion of proteins.
Supplier: Invitrogen™ AM2548
Description
Recombinant Proteinase K is a nonspecific serine protease that is useful for general digestion of proteins.
Proteinase K remains active:
- Over a wide pH range (optimal activity between 6.5 and 9.5)
- Under denaturing conditions (e.g., in the presence of SDS or urea)
- In the presence of metal chelating agents (e.g., EDTA)
- At comparatively high temperatures (optimum digestion temperature is 65°C)
Applications
Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.
Performance and quality testing
Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.
Unit definition
One mAnson unit is described as that amount of enzyme that liberates 1 μmole of Folin-positive amino acid within one minute at 37°C using hemoglobin as a substrate.
Specifications
| 20 mg/mL | |
| Store at -20°C. | |
| 5 x 1.25 mL | |
| Ambion |
| T. album | |
| Dry Ice | |
| Proteinase K | |
| Liquid |
For Research Use Only. Not for use in diagnostic procedures.