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  5. Invitrogen™ Recombinant Proteinase K Solution (20 mg/mL)

Invitrogen™ Recombinant Proteinase K Solution (20 mg/mL)

Numéro de catalogue. AM2548
Encompass
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1.25 mL
5 x 1.25 mL
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AM2548 5 x 1.25 mL
AM2546 1.25 mL
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Numéro de catalogue. AM2548 Fournisseur Invitrogen™ Code fournisseur AM2548
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Description

Recombinant Proteinase K is a nonspecific serine protease that is useful for general digestion of proteins.

Recombinant Proteinase K is a nonspecific serine protease that is useful for general digestion of proteins.

Proteinase K remains active:

  • Over a wide pH range (optimal activity between 6.5 and 9.5)
  • Under denaturing conditions (e.g., in the presence of SDS or urea)
  • In the presence of metal chelating agents (e.g., EDTA)
  • At comparatively high temperatures (optimum digestion temperature is 65°C)

Applications

Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.

Performance and quality testing

Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.

Unit definition

One mAnson unit is described as that amount of enzyme that liberates 1 μmole of Folin-positive amino acid within one minute at 37°C using hemoglobin as a substrate.

Order Info

Shipping Condition: Dry Ice

Spécifications

Concentration 20 mg/mL
Host Species T. album
Content And Storage Store at -20°C.
Shipping Condition Dry Ice
Quantity 5 x 1.25 mL
Product Type Proteinase K
Product Line Ambion
Form Liquid

For Research Use Only. Not for use in diagnostic procedures.