Learn More
Invitrogen™ Formamide (Deionized)
Description
Ambion formamide has been deionized, is nuclease-free, and comes packaged as a liquid in an amber glass bottle under argon. Supplied in 5 bottles containing 500 g each. Formamide lowers the melting temperature of nucleic acid duplexes and is widely used in applications such as Southern and northern blotting, nuclease protection assays, S1 mapping, and in situ hybridization. For critical applications, it is recommended that the formamide from previously opened bottles be deionized before use.
Appearance: Clear, colorless
Molecular Weight: 45.04
Purity: ≥99.5%
Quality Control: The product has been tested for RNase and DNase contamination.
Order Info
Shipping Condition: Room temperature
Specifications
Specifications
| Color | Colorless |
| Content And Storage | Store at 4° or -20°C as small aliquots. |
| Packaging Type | Bottle |
| Product Type | Formamide |
| Product Line | Ambion |
| Purity | >99.5% |
| Quantity | 5 x 500 g |
| Form | Liquid |
Frequently Asked Questions (FAQs)
For long-term storage, we recommend resuspending the RNA in stabilized formamide and storing at -70 degrees C. To remove the formamide, add 4 volumes ethanol and, if less than 20 µg RNA, also add NaCl to 0.2 M. Precipitate the RNA and use for downstream experiments.
There are many sample buffer formulations used, however we have found a distinct difference in the band appearance depending on the sample buffer composition. After evaluating urea, formamide, and various buffer systems, we found that the sharpest, flattest bands were obtained with a urea, Ficoll, and TBE buffer solution. Sample buffers made with formamide resulted in fuzzy, indistinct bands.
For Research Use Only. Not for use in diagnostic procedures.