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Invitrogen™ Ambion™ Cells-to-CT™ 1-Step TaqMan™ Kit DFS Item

Perform expression analysis directly from cultured cells without RNA purification

$364.35 - $6,510.00

Specifications

Detection Method Primer-Probe Detection
For Use With (Application) Gene Expression Analysis and Genotyping, Real Time PCR-Based Gene Expression Profiling
Storage Requirements Store Lysis Solution at 2° to 8°C. Store DNase, Stop Solution, and TaqMan qRT-PCR Mix at -5° to -30°C.
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 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. No. of Tests Price Quantity    

A25603

DFS Item
 
life technologies
A25603
100.0 Each for $1,797.60

A25602

DFS Item
 
life technologies
A25602
400.0 Each for $6,510.00

A25605

DFS Item
 
life technologies
A25605
20.0 Each for $364.35
Description & Specifications

Specifications

Detection Method Primer-Probe Detection
For Use With (Application) Gene Expression Analysis and Genotyping, Real Time PCR-Based Gene Expression Profiling
Storage Requirements Store Lysis Solution at 2° to 8°C. Store DNase, Stop Solution, and TaqMan qRT-PCR Mix at -5° to -30°C.

Cells-to-CT 1-Step TaqMan Kit is a single-tube format of the RT-PCR step streamlines the “sample to answer” workflow. Combining the simple Cells-to- CT lysis technology with the fast cycling capability of the 1-step RT-PCR master mix allows quicker time to results with limited sample handling.

  • Complete: optimized workflow includes cell lysis reagents with gDNA removal and TAqMan 1-Step RT-PCR Master Mix
  • Faster than ever: 7-minute sample prep, including DNase treatment, combined with a 28-minute real-time RT-PCR step allows for approx. 35 minute sample-to-answer workflow
  • Simple: 10 steps total (sample prep and real-time RT-PCR combined)
  • Robust: perform gene expression analysis on 10-100,000 cells per sample; results equivalent to those from purified RNA
  • Convenient: contains sufficient reagents to perform five real-time RT-PCRs for each cell sample you prepare; this allows you to study multiple targets or perform replicate RT-PCRs

TAqMan primer/probe sets sold separately.
The TAqMan Cells-to-CT Control Kit was designed for use with TAqMan Cells-to-CT products. This kit contains an endogenous control (ACTB) to normalize for sample loading variability and an artifical XenoRNA™ control and corresponding TAqMan Gene Expression assay to monitor the effects of PCR inhibition. For use with 7500 System, 7900HT Fast System, StepOnePlus System, QuantStudio Real-Time PCR Systems, Thermal Cyclers StepOne System, ViiA 7 System, 7500 Fast Dx System, 7500 Fast System and 7900HT System.

Gene Expression Analysis and Genotyping, Real Time PCR-Based Gene Expression Profiling