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  5. Invitrogen™ Alexa Fluor™ 647-Poly-GT Peptide Substrate

Invitrogen™ Alexa Fluor™ 647-Poly-GT Peptide Substrate

Catalog No. PV5693
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10 nmol
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PV5693 1 nmol
PV5836 10 nmol
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Catalog No. PV5693 Supplier Invitrogen™ Supplier No. PV5693
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Description

A general AlexaFluor647-labeled peptide substrate consisting of a polymer of glutamate, and tyrosine that is used to monitor tyrosine kinase acitivty.

A general AlexaFluor647-labeled peptide substrate consisting of a polymer of glutamate, and tyrosine that is used to monitor tyrosine kinase acitivty.

For use in combination with the LanthaScreen™ Eu-PY20 Antibody (Cat. No. PV5696) to evaluate inhibitors in medium to high throughput screening applications.

Specifications

Assay Entry Biochemical europium phospho-specific activity
Color Far-Red
Content And Storage 30 μM AlexaFluor 647 Poly-GT peptide substrate in 10 mM HEPES, 137 mM NaCl, 2.7 mM KCl, pH 7.5 (HEPES buffered saline).

Store at -20°C and protect from light.
Detection Method Fluorescence
Form Liquid
Product Line LanthaScreen
Quantity 1 nmol
Substrate Random (Glu:Tyr) polymer in a ratio of 4:1
Substrate Properties Peptide-Based Substrate
Substrate Type Kinase Substrate
Target Enzyme Tyrosine Kinases
Excitation/Emission 340/665 nm
Label or Dye Alexa Fluor™ 647
Shipping Condition Dry Ice
Target Entry ABL1 G250E, CSF1R, CSK, EPHA5, KIT, PDGFRA V561D, SYK, TYRO3, ZAP70, FGFR1, FGFR2, EGFR, EPHB2, FGFR4, IGF1R, LYNA, MATK, MERTK, NTRK3, RET V804L, TEK, FLT1, MUSK, SRCN1, ABL1 E255K, EPHA4, FYN, PTK2, PTK2B, RET, ABL1 T315I, EPHA3, EPHB4, FLT3, FLT4, INSR, LCK, YES1, EGFR L858R, ERBB4, KDR, MET, TNK2, ABL1, ABL2, AXL, FGFR3, FGR, HCK, KIT V654A, EPHA1, EPHA8, EPHB3, INSRR, LYNB, NTRK1, PDGFRA D842V, SRMS, EGFR L861Q, EPHA2, FER, LTK, PDGFRA, EPHA7, EPHB1, FRK, ITK, FLT3 D835Y, SRC, BTK, FES, ERBB2, NTRK2
Technique TR-FRET
Validated Kinases EGFR
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For Research Use Only. Not for use in diagnostic procedures.