Molecular Probes™ Alexa Fluor™ 633 Hydrazide 

Description

Alexa Fluor™ 633 Hydrazide is useful as a cell tracer and as a reactive dye for labeling aldehydes or ketones in polysaccharides or glycoproteins. Alexa Fluor™ 633 is a bright, far red fluorescent dye with excitation ideally suited to the 633 nm laser line. Used for stable signal generation in imaging and flow cytometry, Alexa Fluor™ 633 dye is water soluble and pH-insensitive from pH 4 to pH 10.

To make a concentrated stock solution from 1–10 mM, dissolve the dye-hydrazide in either H₂O or DMSO. Store frozen and protected from light.

Detailed information about this AlexaFluor™ hydrazide:
• Fluorophore label: Alexa Fluor™ 633 dye
• Reactive group: hydrazide
• Reactivity: Aldehydes or keytones in polysaccharides or glycoproteins
• Ex/Em of the conjugate: 624/643 nm
• Extinction coefficient: 110,000 cm-1M-1
• Spectrally similar dyes: Cy5
• Molecular weight: ∼1,150

Cell Tracking and Tracing Applications
Alexa Fluor™ hydrazides and hydroxlamines are useful as low molecular weight, membrane-impermeant, aldehyde-fixable cell tracers, exhibiting brighter fluorescence and greater photostability than cell tracers derived from other spectrally similar fluorophores. They are easily loaded into cells by microinjection, infusion from patch pipette, or uptake induced by our Influx™ Pinocytic Cell-Loading Reagent (Cat. No. I14402).

Glycoprotein and Polysaccharide Labeling Applications
The Alexa Fluor™ hydrazides and hydroxlamines are reactive molecules that can be used to add a fluorescent label to biomolecules containing aldehydes or ketones. Aldehydes and ketones can be introduced into polysaccharides and glycoproteins by periodate-mediated oxidation of vicinal diols. Galactose oxidase can also be used to oxidize terminal galactose residues of glycoproteins to aldehydes.

Hydrazide vs Hydroxylamine
Hydrazine derivatives react with ketones and aldehydes to yield relatively stable hydrazones. Hydroxylamine derivatives (aminooxy compounds) react with aldehydes and ketones to yield oximes. Oximes are superior to hydrazones with respect to hydrolytic stability. Both hydrazones and oximes can be reduced with sodium borohydride (NaBH₄) to further increase the stability of the linkage.

Learn More About Protein and Antibody Labeling
We offer a wide selection of Molecular Probes™ antibody and protein labeling kits to fit your starting material and your experimental setup. See our Antibody Labeling kits or use our Labeling Chemistry Selection Tool for other choices. To learn more about our labeling kits, read Kits for Labeling Proteins and Nucleic Acids—Section 1.2 in The Molecular Probes™ Handbook.

We’ll Make a Custom Conjugate for You
If you can’t find what you’re looking for in our online catalog, we’ll prepare a custom antibody or protein conjugate for you. Our custom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 13485:2000 certified.

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DMSO (dimethylsulfoxide) (Cat. No. D12345)
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Antibody Conjugate Purification Kit for 20-50 μg (Cat. No. A33087)
Antibody Conjugate Purification kit for 50-100 μg (Cat. No. A33088)