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Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents
AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.
$308.00 - $2490.00
Specifications
Description | Works on all AB platforms, but is optimized for the 7500. |
---|---|
Detection Method | Primer-probe |
Format | Tube |
GC-Rich PCR Performance | High |
PCR Method | 1-step RT-qPCR |
Catalog Number | Mfr. No. | No. of Reactions | Price | Quantity | |||||
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Catalog Number | Mfr. No. | No. of Reactions | Price | Quantity | |||||
AM1005
|
Applied Biosystems™
AM1005 |
100 Reactions |
Each for $308.00
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4387424
|
Applied Biosystems™
4387424 |
500 Reactions |
Each for $1,474.00
|
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4387391
|
Applied Biosystems™
4387391 |
1000 Reactions |
Each for $2,490.00
|
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Description
- Recommended for RNA pathogen amplification
- Consistent amplification of RNA targets with high specificity and sensitivity
- Optimized to work with your target-specific primers and probes
- Contains ROX for quantitative fluorescent signal normalization
AgPath-ID™ One-Step RT-PCR Reagents are configured for fast and simple reaction setup. The reactions are assembled in a single tube, minimizing sample handling errors and expediting set-up time. Once assembled, results are available in approximately one hour. The 25X RT-PCR Enzyme Mix included in the kit contains highly efficient ArrayScript™ Reverse Transcriptase, a mutant MMLV RT that produces high cDNA yields, and AmpliTaq Gold™ polymerase, the preferred hot-start DNA polymerase for specific target amplification. The 2X RT-PCR Buffer has been optimized for efficient, robust reverse transcription and PCR includes the passive reference dye, ROX™ Dye, for quantitative fluorescent signal normalization. A Detection Enhancer is also provided as an optional reagent for amplification of templates with a high GC content or persistent secondary structure.
Specifications
Works on all AB platforms, but is optimized for the 7500. | |
Tube | |
1-step RT-qPCR | |
Standard | |
ROX (Pre-mixed) | |
One-Step RT-PCR Reagent | |
Dry Ice | |
25 X |
Primer-probe | |
High | |
AmpliTaq Gold DNA Polymerase | |
7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex | |
AgPath-ID™, Ambion™ | |
ArrayScript™ | |
25X | |
RNA |
For Veterinary Use Only.