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Thermo Scientific™ ActivX™ Fluorophosphonate Probes


Tagged phosphonate probes to assay serine hydrolase active sites using fluorescence, biotin-affinity purification or detection, or mass spectrometry.

Manufacturer: thermo scientific™  88317

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Catalog No. PI88317

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Description & Specifications

Specifications

Description ActivX Desthiobiotin-FP Serine Hydrolase Probe
Formulation Desthiobiotin-fluorophosphonate, MW 464
Quantity 4.6µg
Sufficient For 100 labeling reactions or 10 pull-down assays

Thermo Scientific™ ActivX Fluorophosphonate (FP) Probes allow covalent labeling of the serine hydrolase active site with affinity, fluorescent or reactive tags to facilitate selective enrichment, detection or multiplex labeling.

ActivX FP Probes feature a reactive fluorophosphonate group that specifically and covalently labels the active-site serine of enzymatically active serine hydrolases. These probes are available with a desthiobiotin (biotin analog) tag for selective enrichment, TAMRA fluorophore for detection or a reactive- azido group (Staudinger reagents) that facilitates multiplex labeling when used with phosphine- or alkyne-derivatized tags. These probes can be used to assess activity or screen small molecule inhibitors against enzymes derived from cell lysates, subcellular fractions, tissues and recombinant proteins.

Highlights:

  • Specific – labels the reactive site of active serine hydrolases

  • Compatible – tags available for capture, detection and Staudinger conjugation

  • Flexible – use for in vitro or intracellular enzyme labeling

Recommended for:

Determine serine hydrolase enzyme activity in cells and lysates; Mapping the active-site serine of functionally diverse serine hydrolase family members (e.g. proteases, lipases, esterases); Screen for small molecule binding affinities and active-site inhibition; Profile serine hydrolases using fluorescent, Western blot or mass spectrometry workflows