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Thermo Scientific™ Acclaim™ HILIC-10 HPLC Columns

Catalog No. p-4522896
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Length (Metric):
10 mm
150 mm
Diameter (Metric):
2.1 mm
Particle Size:
3 μm
5 μm
Catalog No. Length (Metric) Diameter (Metric) Particle Size
DX074259 150 mm 2.1 mm 3 μm
DX074263 10 mm 2.1 mm 5 μm
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Catalog No. DX074259

Supplier: Thermo Scientific™ 074259

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Use this HILIC column to retain and separate highly polar molecules that are unretained by reversed-phase chromatography.

Separate highly hydrophilic molecules by hydrophilic interaction liquid chromatography using Thermo Scientific™ Acclaim™ HILIC-10 LC Columns. These columns retain and separate highly polar molecules that are unretained by reversed-phase chromatography. Their high-purity, spherical, porous silica base is covalently modified with a proprietary hydrophilic layer. This chemistry enables the use of 5–20% aqueous mobile phase while maintaining affinity for polar analytes. Sample throughput is increased without the need to evaporate and reconstitute samples dissolved in nonaqueous solvents.

Rugged and Reproducible Separations of Highly Polar Compounds:
  • Unique selectivity, complementary to reversed-phase columns
  • Enhanced sensitivity for MS detection
  • Hydrolytically stable
  • Rugged column packing
  • Broad application range, including separation of hydrophilic drugs and drug metabolites
Acclaim HILIC-10 LC columns are available in 3μm particle size and a variety of column formats: 4.6×150mm, for high-throughput routine analysis; 3.0×150mm, for high-throughput analysis with reduced solvent consumption; and 2.1×150mm, for LC-MS analysis.

Specifications

Column Chemistry Proprietary Polar Group
Column Format Analytical Column
Diameter (Metric) 2.1 mm
Flow Rate 0.2 to 0.4 mL/min
For Use With LC/MS
Length (Metric) 150 mm
Max. Pressure 8500 psi (590 bar)
Particle Size 3 μm
pH 2 to 8
Pore Size 120 Å
Quantity Each
Surface Area 300 m²/g
Temperature 70°C
Stationary Phase Proprietary
Product Line Acclaim
Packing Material Spherical, Fully Porous, Ultrapure Silica
Carbon Load 8%
Column Type HILIC
Endcapped Yes
Phase HILIC
Type Analytical Column
Green Features Responsibly packaged
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Limited warranty period: Subject to all warranty requirements and exclusions set out in our Terms and Conditions of Sale, this product is warranted from the date we ship the product and for ninety (90) days thereafter. Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.