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Promega Access RT-PCR System

Catalog No. PRA1260
Encompass
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Kit Type:
Access RT-PCR introductory system
Access RT-PCR system
No. of Reactions:
100 Reactions
20 Reactions
500 Reactions
3 product options available for selection
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Catalog No. Kit Type No. of Reactions
PRA1260 Access RT-PCR introductory system 20 Reactions
PRA1250 Access RT-PCR system 100 Reactions
PRA1280 Access RT-PCR system 500 Reactions
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Catalog No. PRA1260 Supplier Promega Supplier No. A1260

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

Access RT-PCR System is designed for the reverse transcription and PCR amplification of a specific target RNA from total RNA or mRNA.

  • Convenient RT-PCR in One Tube
  • All the reagents you need for single-tube reverse transcription and PCR
  • Separate components allow you to control every step of the reaction

Specifications

Amplicon Size 323
Content And Storage

Contains sufficient reagents for 20 reactions
• Positive Control RNA and Primers
• AMV Reverse Transcriptase, 100 μL
• Tfl DNA Polymerase, 100 μL
• AMV/Tfl 5X reaction Buffer, 1 mL
• MgSO, 1.25 mL
• dNTP Mixture, 20 μL
• 50 μL Positive Control

Store at –30° to –10°C
Control Template Positive Control RNA and Primers
Form Liquid
Format Kit
PCR Method RT-PCR
Polymerase Tfl DNA Polymerase
Technique RT-PCR
DNase DNase-free
Host Cell Avian Myeloblastosis Virus, Thermus flavus
Includes Label or Dye No
Kit Type Access RT-PCR introductory system
No. of Tests 20 Test
Primer Control Primers
Product Line Access
Product Type RT-PCR Kit
Quantity 20 Reactions
Research Category Gene Expression Analysis
Reverse Transcriptase AMV Reverse Transcriptase
Ribonuclease H Activity Yes
Sufficient For 20 Reactions
For Use With (Application) RT-PCR
Activation Time 2 min.
Compatibility Total RNA, mRNA
Final Product Type cDNA
Hot Start No
No. of Reactions 20 Reactions
Overhang 3'-A
Reaction Format Separate Components
Reagent Type Upstream Control Primer, Downstream Control Primer, Positive Control RNA with Carrier, AMV Reverse Transcriptase, AMV/Tfl 5X Reaction Buffer, dNTP Mix, MgSO4, Tfl DNA Polymerase, Nuclease-Free Water
Sample Type Total RNA, mRNA
Size (Final Product) Up to 5 kb
Starting Material Total RNA or mRNA
Source Avian Myeloblastosis Virus (AMV), Thermus flavus
Storage Buffer AMV/Tfl 5X Reaction Buffer
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