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  5. Thermo Scientific™ AccelerOme™ Label-Free MS Sample Prep Kit

Thermo Scientific™ AccelerOme™ Label-Free MS Sample Prep Kit

Catalog No. PIA50944
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1 x 16 Reactions
1 x 36 Reactions
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PIA50944 1 x 16 Reactions
PIA50945 1 x 36 Reactions
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Catalog No. PIA50944 Supplier Thermo Scientific™ Supplier No. A50944
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Description

Kit provides ready-to-go reagents for efficient and robust proteomics mass spectrometry (MS) sample preparation for use with the AccelerOme Automated Sample Prep System.

  • Kit contains pre-formulated buffers, MS-grade trypsin/Lys-C enzyme mix, and peptide clean up cartridges for the processing of samples from protein lysis and digestion through peptide clean-up
  • The single-use components are conveniently provided in labeled trays for direct placement on the AccelerOme system
  • A peptide retention time calibration standard is also included with the kit (shipped separately) that can be spiked into the sample before LC-MS analysis to assess system performance.
  • Complete set of ready-to-use proteomic sample prep and clean-up reagents for easy loading onto instrument
  • Reagents combined with AccelerOme ecosystem provide consistent, high-quality results with technical replicates with <5% CV
  • Reagents verified on system using cells, plasma, proteins, and tissue samples for 1–100 μg samples
  • Instrumentation and reagents perform the work enabling time away from tedious hands-on sample preparation

Specifications

Product Type Reagent Kit
Product Line Thermo Scientific
Starting Material Protein Samples
Detection Method UV Absorption
Quantity 1 x 16 Reactions

Frequently Asked Questions (FAQs)

Is the AccelerOme Label-Free MS Sample Prep Kit compatible with the BCA protein assay?

Yes, the buffer in the AccelerOme Label-Free MS Sample Prep Kit, in which samples are resuspended, is compatible with the BCA protein assay.

Is the Lysis Buffer (Cat. No. A45735) compatible with the AccelerOme Label-Free MS Sample Prep Kit?

The Lysis Buffer (Cat. No. A45735) has been designed to be used with manual EasyPep MS Sample Prep Kits. The format of the Lysis Buffer container is not compatible with the AccelerOme platform and therefore it cannot be used with the AccelerOme Label-Free MS Sample Prep Kit.

Can I use the samples obtained with the AccelerOme Label-Free MS Sample Prep Kit directly for mass spectrometry?

Yes, all buffers used in the AccelerOme Label-Free MS Sample Prep Kit are MS-compatible, and the samples can be directly used for mass spectrometry.
Can the AccelerOme Label-Free MS Sample Prep Kit be reused?

The AccelerOme Label-Free MS Sample Prep Kit is a single-use kit, and it cannot be reused.

Can I use the AccelerOme Label-Free MS Sample Prep Kit without the AccelerOme platform?

The AccelerOme Label-Free MS Sample Prep Kit has been developed to be used in the AccelerOme Automated Sample Preparation Platform. If you do not have the AccelerOme Automated Sample Preparation Platform, we recommend using EasyPep MS Sample Prep Kits (Cat. No. A40006, A57864, A45733 or A45734, depending on the number of reactions you would like to perform).

Which type of samples can be used with the AccelerOme Label-Free MS Sample Prep Kit?

The AccelerOme Label-Free MS Sample Prep Kit was initially optimized for proteins extracted from plasma, serum, cells, and tissue. However, it has also been demonstrated to work with bacteria and yeast samples. The AccelerOme Label-Free MS Sample Prep Kit allows working with samples containing between 1 to 100 µg of protein.

How should I use the AccelerOme Label-Free MS Sample Prep Kit in the AccelerOme Automated Sample Preparation Platform?

All the buffers, reagents and other components in the AccelerOme Label-Free MS Sample Prep Kit come in a ready-to-use tray that has to be positioned into the AccelerOme Automated Sample Preparation Platform. Once placed on the device, the rest of the procedure is automated.