Thermo Scientific™ 1-Step Human High-Yield IVT Kit

The 1-Step High-Yield IVT System uses modified HeLa cell extracts to take advantage of the robust human transcription and translation machinery and generate functional full-length proteins. In this system, protein expression is performed in a proprietary mini dialysis device that allows a continuous supply of nucleotides, amino acids and energy generating substrates into the reaction while removing inhibitors of proteins synthesis. This continuous-exchange cell-free (CECF) system enables protein expression for up to 24 hours and produces protein yields ranging from 250 to 750μg/mL. The complete kits come with all components required for proper transcription and translation of recombinant protein, including an optimized expression vector.

Highlights:

• High expression – up to 750μg/mL of expressed protein

• Reproducibility – low variability between experiments

• Fast – express high levels of protein with an overnight incubation

• Functional – obtain more active protein than with bacterial protein expression systems

1-Step Protein Expression:

• Streamlined reaction – requires only five components

• Easy-to-use – transcription and translation is performed in one reaction step

• Adapt for labeling – amenable to incorporation of heavy or unnatural amino acids

Includes:

Complete kits contain cell lysate, reaction mix, accessory proteins, 5X dialysis buffer, control DNA, cloning vector, mini dialysis devices and centrifuge tubes

Requires:

30°C incubator or water bath

Recommended for:

Production of recombinant proteins for structural analysis; Generate active proteins for protein interaction studies; Perform rapid expression and characterization of mutant proteins; Small-scale production of virual particles; Incorporation of unnatural amino acids or toxic protein production