Restriction Enzymes
Restriction enzymes cleave DNA into fragments at or near specific recognition sequences known as restriction sites; also known as restriction endonuclease, or restrictase. Products include various restriction enzymes, kits, buffers, etc.
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Optimal Reaction Temperature
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Sensitive to Heat Inactivation
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1
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15
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452
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1
| Components | 25 to 50% Water, >50% Glycerin |
|---|---|
| Cut Site | GC.GGCCGC |
| Content And Storage | Keep container tightly closed |
| Form | Liquid |
| Product Type | NotI |
| Storage Buffer | 20mM Tris HCl (pH 7.8 at 25°C), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.02% Triton™ X-100, 0.2mg/mL BSA, 50% Glycerol |
| pH | 7.4 |
| Concentration | 10 U/μL |
| For Use With (Application) | >90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Not I |
2
| Components | 25 to 50% Water, >50% Glycerin, 1 to 2.5% Potassium Chloride |
|---|---|
| Cut Site | A.AGCTT |
| Content And Storage | Keep container tightly closed |
| Form | Liquid |
| Product Type | HindIII |
| Storage Buffer | 10mM Tris HCl (pH 7.5 at 25°C), 250mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA, 50% Glycerol |
| Incubator Temperature | 37°C |
| Concentration | 10 U/μL |
| For Use With (Application) | >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with HaeIII |
5
| Components | >50% Water, 25 to 50% Glycerin |
|---|---|
| Cut Site | AT.CGAT |
| Content And Storage | Keep container tightly closed |
| Form | Liquid |
| Product Type | ClaI (Bsu15I) |
| Storage Buffer | 10mM Tris HCl (pH 8 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/mL BSA, 50% Glycerol |
| pH | 7.4 |
| Incubator Temperature | 37°C |
| Concentration | 10 U/μL |
6
| Components | 10X OPTIZYME™ Buffer 4 |
|---|---|
| Cut Site | CCC.GGG |
| Form | Liquid |
| Product Type | SmaI |
| Storage Buffer | 10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 50% Glycerol |
| pH | 7.4 |
| Incubator Temperature | 30°C |
| Concentration | 10 U/μL |
| For Use With (Application) | >90% of pUC19 DNA fragments can be ligated and re-cut after 50-fold over-digestion with SmaI |
9
| Components | 25 to 50% Water, >50% Glycerin, 1 to 2.5% Sodium Chloride |
|---|---|
| Cut Site | A.GATCT |
| Content And Storage | Keep container tightly closed |
| Form | Liquid |
| Product Type | BglII |
| Storage Buffer | 10mM Tris HCl (pH 8.2 at 25°C), 200mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/mL BSA, 50% Glycerol |
| pH | 7.4 |
| Incubator Temperature | 37°C |
| Concentration | 10 U/μL |
| For Use With (Application) | >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BglII |
12
Thermo Scientific™ FastDigest NdeI
The FastDigest NdeI restriction enzyme recognizes CA^TATG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: FauNDI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Nde I |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
| Overhang | 5' protruding ends |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Inactivation Temperature | 65°C |
| Product Line | FastDigest |
13
Thermo Scientific™ TaiI (MaeII) (10 U/μL)
Cut at ACGT^ sites with TaiI (MaeII) restriction enzyme, which performs best at 65°C in R buffer (Isoschizomers: HpyCH4IV, MaeII).
| Sensitive to Heat Inactivation | No |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | TaiI (MaeII) |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer R |
| Type IIS RE | No |
| Optimal Reaction Temperature | 65°C |
| Concentration | 10 U/μL |
14
Thermo Scientific™ BfmI (SfcI) (10 U/μL)
Cut at C^TRYAG sites with BfmI (SfcI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: BstSFI, SfcI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | BfmI (SfcI) |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
15
Thermo Scientific™ TAE Buffer (Tris-acetate-EDTA) (50X)
Optimize electrophoresis of both genomic and large supercoiled DNA in agarose and polyacrylamide gels with Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA).