Restriction Enzymes

Restriction enzymes cleave DNA into fragments at or near specific recognition sequences known as restriction sites; also known as restriction endonuclease, or restrictase. Products include various restriction enzymes, kits, buffers, etc.

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OPTIZYME™ HpaII, Fisher BioReagents™

Pricing and Availability

Components	25 to 50% Water, >50% Glycerin, 1 to 2.5% Potassium Chloride
Cut Site	A.AGCTT
Content And Storage	Keep container tightly closed
Form	Liquid
Product Type	HindIII
Storage Buffer	10mM Tris HCl (pH 7.5 at 25°C), 250mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
Incubator Temperature	37°C
Concentration	10 U/μL
For Use With (Application)	>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with HaeIII

OPTIZYME™ BpiI (BbsI), Fisher BioReagents™

Pricing and Availability

OPTIZYME™ NotI, Fisher BioReagents™

Pricing and Availability
Specifications

Components	25 to 50% Water, >50% Glycerin
Cut Site	GC.GGCCGC
Content And Storage	Keep container tightly closed
Form	Liquid
Product Type	NotI
Storage Buffer	20mM Tris HCl (pH 7.8 at 25°C), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.02% Triton™ X-100, 0.2mg/mL BSA, 50% Glycerol
pH	7.4
Concentration	10 U/μL
For Use With (Application)	>90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Not I

OPTIZYME™ ClaI (Bsu15I), Fisher BioReagents™

Pricing and Availability
Specifications

Components	>50% Water, 25 to 50% Glycerin
Cut Site	AT.CGAT
Content And Storage	Keep container tightly closed
Form	Liquid
Product Type	ClaI (Bsu15I)
Storage Buffer	10mM Tris HCl (pH 8 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/mL BSA, 50% Glycerol
pH	7.4
Incubator Temperature	37°C
Concentration	10 U/μL

OPTIZYME™ BglII, Fisher BioReagents™

Pricing and Availability
Specifications

Components	25 to 50% Water, >50% Glycerin, 1 to 2.5% Sodium Chloride
Cut Site	A.GATCT
Content And Storage	Keep container tightly closed
Form	Liquid
Product Type	BglII
Storage Buffer	10mM Tris HCl (pH 8.2 at 25°C), 200mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/mL BSA, 50% Glycerol
pH	7.4
Incubator Temperature	37°C
Concentration	10 U/μL
For Use With (Application)	>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BglII

Components	10X OPTIZYME™ Buffer 4
Cut Site	CCC.GGG
Form	Liquid
Product Type	SmaI
Storage Buffer	10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 50% Glycerol
pH	7.4
Incubator Temperature	30°C
Concentration	10 U/μL
For Use With (Application)	>90% of pUC19 DNA fragments can be ligated and re-cut after 50-fold over-digestion with SmaI

Thermo Scientific™ TheraPure™ Restriction Enzymes

Thermo Scientific TheraPure in vitro transcription (IVT) enzymes are designed for advanced research and early development of mRNA therapeutics and vaccines.

Pricing and Availability
Specifications

Cut Site	CTCTTC(1/4)^
Content And Storage	Store at -20°C.
Form	Liquid
Enzyme	Eam1104I
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Quantity	3000 U, 150 μL
Type IIS RE	Yes
Incubator Temperature	37°C
Concentration	20 U/μL
For Use With (Application)	In vitro Transcription
Inactivation Temperature	65°C
Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Research Category	Drug Development
Compatible Buffer	10X Tango Buffer
Optimal Reaction Temperature	37°C
Product Line	TheraPure

Thermo Scientific™ EcoO109I (DraII) (10 U/μL)

Cut at RG^GNCCY sites with EcoO109I (DraII) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: DraII).

Pricing and Availability

Invitrogen™ Anza™ 7 BshTI

Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.

Pricing and Availability
Specifications

Shipping Condition	Approved for shipment on Wet or Dry Ice
Sensitive to Heat Inactivation	No
Product Type	Restriction Enzyme Cloning System
Enzyme	BshTI
Methylation Sensitivity	CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	Anza™ 10X Red Buffer, Anza™ 10X Buffer
Type IIS RE	No
Optimal Reaction Temperature	37°C
Product Line	Anza

Restriction Enzymes

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