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Bioluminescence Assays and Reagents

Luciferase vectors, cell lysis buffers, signal enhancers, transfection reagents, assay kits, and other associated products for optimal luciferase reporter assay performance.
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1

Invitrogen™ D-Luciferin, Potassium Salt  DFS Item

Needed for luciferin/luciferase light-producing reaction

2

Invitrogen™ D-Luciferin, Sodium Salt, 25 mg  DFS Item

Part of the luciferin/luciferase light-producing reaction

3

Thermo Scientific™ Pierce™ Cypridina-Firefly Luciferase Dual Assay Kit

Simultaneously detect intracellular Cypridina and Red Firefly luciferase activity in mammalian whole cell lysates.

Substrate Vargulin (Cypridina Luciferin), D-Luciferin
Assay Reporter Enzyme, Luciferase Reporter Assay
Substrate Properties Chemical Substrate
Target Luciferase, Cypridina Luciferase
Type Assay Kit
Format 384-well plate, 96-well plate
For Use With (Equipment) Luminometer (Microplate)
Compatible Cells Mammalian Cells
Substrate Type Luciferase Substrate
Technique Enhanced Chemiluminescence
Label Type Enzyme Labeled
Product Line Pierce
Detection Method Bioluminescence
4

Thermo Scientific™ Pierce™ Firefly Luciferase Glow Assay Kit

Obtain bright and stable bioluminescence signal (half-life greater than 2 hours) in the presence of red firefly luciferase reporters

Substrate D-Luciferin
Assay Reporter Enzyme, Luciferase Reporter Assay
Substrate Properties Chemical Substrate
Target Luciferase, Firefly Luciferase
Type Assay Kit
Format 384-well plate, 96-well plate
For Use With (Equipment) Luminometer (Microplate)
Compatible Cells Mammalian Cells
Substrate Type Luciferase Substrate
Technique Enhanced Chemiluminescence
Label Type Enzyme Labeled
Product Line Pierce
Detection Method Bioluminescence
5

Thermo Scientific™ Pierce™ Firefly Luciferase Flash Assay Kit

Assay regulatory element transcriptional activity in whole cell lysates with this highly sensitive flash-based firefly luciferase kit.

Substrate D-Luciferin
Assay Reporter Enzyme, Luciferase Reporter Assay
Substrate Properties Chemical Substrate
Target Luciferase, Firefly Luciferase
Type Assay Kit
Format 384-well plate, 96-well plate
For Use With (Equipment) Luminometer (Microplate)
Compatible Cells Mammalian Cells
Substrate Type Luciferase Substrate
Technique Enhanced Chemiluminescence
Label Type Enzyme Labeled
Product Line Pierce
Detection Method Bioluminescence
6

Thermo Scientific™ Pierce™ Gaussia-Firefly Luciferase Dual Assay Kit

Simultaneously detect intracellular Gaussia and Red Firefly luciferase activity in mammalian whole cell lysates.

Substrate Coelenterazine, D-Luciferin
Assay Reporter Enzyme, Luciferase Reporter Assay
Substrate Properties Chemical Substrate
Target Luciferase, Firefly Luciferase, Gaussia Luciferase
Type Assay Kit
Format 384-well plate, 96-well plate
For Use With (Equipment) Luminometer (Microplate)
Compatible Cells Mammalian Cells
Substrate Type Luciferase Substrate
Technique Enhanced Chemiluminescence
Label Type Enzyme Labeled
Product Line Pierce
Detection Method Bioluminescence
7

Thermo Scientific™ Pierce™ Renilla-Firefly Luciferase Dual Assay Kit

Simultaneously detect intracellular Renilla and Red Firefly luciferase activity in mammalian whole cell lysates.

Substrate Coelenterazine, D-Luciferin
Assay Reporter Enzyme, Luciferase Reporter Assay
Substrate Properties Chemical Substrate
Target Luciferase, Firefly Luciferase, Green Renilla Luciferase
Type Assay Kit
Format 384-well plate, 96-well plate
For Use With (Equipment) Luminometer (Microplate)
Compatible Cells Mammalian Cells
Substrate Type Luciferase Substrate
Technique Enhanced Chemiluminescence
Label Type Enzyme Labeled
Product Line Pierce
Detection Method Bioluminescence
8

Thermo Scientific™ Pierce™ Firefly Luc One-Step Glow Assay Kit

Perform firefly luciferase assays in high-throughput screening applications.

Substrate D-Luciferin
Assay Reporter Enzyme, Luciferase Reporter Assay
Substrate Properties Chemical Substrate
Target Luciferase, Firefly Luciferase, Cypridina Luciferase
Type Assay Kit
Format 384-well plate, 96-well plate
For Use With (Equipment) Luminometer (Microplate)
Compatible Cells Mammalian Cells
Substrate Type Luciferase Substrate
Technique Enhanced Chemiluminescence
Label Type Enzyme Labeled
Detection Method Bioluminescence