Perform high-throughput protein recovery (7K MWCO) and sample desalting for mass spectrometry, HPLC, capillary electrophoresis, metabolite screening, and assay development with ready-to-use Thermo Scientific Pierce Zeba desalting chromatography columns, plates, and cartridges.
Supplier: Thermo Scientific™ 89935
Perform high-throughput protein recovery and sample desalting for mass spectrometry, HPLC, capillary electrophoresis, metabolite screening, and assay development with ready-to-use Pierce Zeba desalting chromatography columns, plates, and cartridges. Rapidly desalt protein and biological samples for manual or automated liquid chromatography (LC) with ≥95% retention of salts and other small molecular contaminants (<1,000 Da).
Zeba Spin Desalting columns, plates, and chromatography cartridge fittings are convenient, ready-to-use devices compatible with major automated liquid-chromatography systems or for manual syringe processing. The polypropylene columns have a 7K MWCO and contain a proprietary high-performance size-exclusion chromatography resin (Zeba High-Performance Resin) that offers excellent protein desalting and recovery in a centrifuge format. When using Zeba Spin Desalting Columns, even very dilute (25 μg/mL) protein samples can be successfully processed to obtain greater than 95% retention (removal) of salts and other small molecules (<1000 MW), and good recovery of proteins and other macromolecules (>7000 MW).
Similarly, Zeba Desalting plates (7K MWCO) are polypropylene devices that contain Zeba High-Performance Resin and provide excellent protein desalting performance, with high recovery of proteins >7000 Da in a centrifuge format for small sample volumes. The plates enable fast, consistent, well-to-well and plate-to-plate reproducibility for small-scale, high-throughput separations. Zeba 96-well spin desalting plates require no resin dispensing or hydration and provide the same high protein recovery as spin columns with at least 95% removal of salts and other small molecules. One plate can process 96 small sample volumes (20 to 100 μL) in just five minutes, offering rapid and exceptional protein desalting.
The Zeba Desalting Chromatography Cartridges contain Zeba High-Performance Resin for desalting or buffer exchange applications. This resin is ideal for removing low molecular weight compounds including salts, fluorescent dyes, biotin, and other small labeling reagents. Protein samples can be processed with unsurpassed protein recovery and 95% retention of salts and other small molecular contaminants (<1,000 Da). Zeba Desalting cartridges provide fast, easy, and reproducible chromatographic separations and can be regenerated for multiple uses.
Benefits of Zeba Spin Desalting columns, plates, and cartridges include:
• High recovery—low-binding resin maximizes protein recovery
• Fast—no fraction screening or waiting for protein to emerge by gravity flow
• Economical—great performance at lower cost than other commercially available cartridges
• Easy-to-use—no cumbersome column preparation or equilibration
• Flexible—available in spin columns, filter spin plates and cartridges for a range of needs
Available formats
• Spin columns—75 μL, 0.5 mL (microcentrifuge), 2 mL, 5 mL, 10 mL columns
• 96-well spin plates—pre-dispensed 96-well filter plates, compatible with centrifugation for manual or automated purification
• Chromatography cartridges—compatible with automated liquid chromatography systems and with syringe processing
| Desalting Chromatography Cartridge | |
| Chromatography Cartridge | |
| Size-exclusion, Proprietary Resin | |
| Zeba™ | |
| 5 mL |
| Upon receipt store at 4–8°C. | |
| Buffer Exchange, Protein | |
| 7.0 kDa | |
| 1 column x 5 mL | |
| 0.1 to 1.5 mL |
