A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format.
Supplier: Thermo Scientific™ PV4305
A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon LAT, containing Tyr-200.
| Z'-LYTE™ Tyrosine 7 Peptide Kinase Assay Kit, 800 x 20μL, 800rxns, Liquid, Tyrosine Kinase Assay Type, Peptide-Based Substrate, 535nm Emission, 405/465nm Excitation, Methylcoumarin Label, End Point Readout, Threonine Kinases Target Enzyme, FRET Technique, Used for Pharma and Biopharma, Protein Assays and Analysis | |
| Liquid | |
| 800 x 20μL | |
| Biochemical phospho-specific activity (no antibody) | |
| Z'-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. See COA for specific storage instructions Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions. | |
| 384-well Plate | |
| FRET | |
| 800 | |
| Biochem. J. 356 p 461-71 (2001) | |
| Based on LAT, Residues 195 to 208, Centering Y-200 (ESIDDYVNVPESGES) | |
| ZAP70 | |
| ZAP70 |
| Z Ì-LYTE™ | |
| Z'-LYTE™ Ser/Thr 11 peptide, Z'-LYTE™ Ser/Thr 11 phospho-peptide, 5X kinase buffer A, ATP, development reagent B, development buffer | |
| Tyrosine Kinase Assay | |
| Z'-LYTE™ Tyrosine 7 Peptide Kinase Assay Kit | |
| 800 x 20 μL Assays | |
| 405/465, 535 nm | |
| Methylcoumarin | |
| End Point | |
| Dry Ice | |
| Peptide-Based Substrate | |
| Tyrosine Kinases |
For Research Use Only. Not for use in diagnostic procedures.
