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Thermo Scientific™ Z'-LYTE™ Kinase Assay Kit - Tyrosine 5 Peptide

Catalog No. PV3676
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800 x 20μL, 800 x 20 μL Assays
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PV3676 800 x 20μL, 800 x 20 μL Assays
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Catalog No. PV3676 Supplier Thermo Scientific™ Supplier No. PV3676
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Description

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format.

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon an optimal peptide substrate for ErbB2.

Specifications

Description Z'-LYTE™ Tyrosine 5 Peptide Kinase Assay Kit, 800 x 20μL, 800rxns, Tyrosine Kinase Assay Type, Peptide-Based Substrate, 535nm Emission, 405/465nm Excitation, Methylcoumarin Label, End Point Readout, Threonine Kinases Target Enzyme, FRET Technique,
Product Line Z ́-LYTE
For Use With (Application) Pharma and biopharma, protein biology, drug discovery and development, target and lead identification and validation, kinase biology, biochemical kinase assays, z and acute-lyte™ kinase assays, enzyme and protein activity assays, protein assays and, FRET
Includes Z'-LYTE™ Ser/Thr 11 peptide, Z'-LYTE™ Ser/Thr 11 phospho-peptide, 5X kinase buffer A, ATP, development reagent B, development buffer
Sample Volume 800 x 20μL
Assay Tyrosine Kinase Assay
Assay Entry Biochemical phospho-specific activity (no antibody)
Content And Storage –80°C, Z'-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.
Product Type Z'-LYTE™ Tyrosine 5 Peptide Kinase Assay Kit
Quantity 800 x 20μL, 800 x 20 μL Assays
Format 384-well plate
Excitation/Emission 405/465, 535 nm
Label or Dye Methylcoumarin
No. of Reactions 800 Reactions
Readout End Point
References Cancer Letters, vol 160, pg 159 (2000)
Shipping Condition Dry Ice
Source ErbB2 Substrate (AAEEIYAARRG)
Substrate Properties Peptide-Based Substrate
Target Entry FER, TEK
Target Enzyme Tyrosine Kinases
Validated Kinases FER, TEK
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For Research Use Only. Not for use in diagnostic procedures.