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Invitrogen™ Z'-LYTE™ Kinase Assay Kit - Tyrosine 4 Peptide

Catalog No. PV3193
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800 x 20 μL Assays
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PV3193 800 x 20 μL Assays
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Catalog No. PV3193 Supplier Invitrogen™ Supplier No. PV3193
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Description

Z'-LYTE™ Kinase Assay Kit - Tyrosine 4 Peptide

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide contains an X-(E⁄D)-Y-(I⁄L⁄V) consensus sequence, where X is any residue.

Specifications

Product Line Z´-LYTE
Target Tyrosine Kinases
For Use With (Application) FRET
Assay Tyrosine Kinase Assay
Content And Storage Z´-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.
Assay Entry Biochemical phospho-specific activity (no antibody)
Label or Dye Methylcoumarin
Product Type Kinase Assay Kit
Quantity 800 x 20 μL Assays
Format 384-well plate
Excitation/Emission 405⁄465, 535
No. of Reactions 800 Reactions
Readout End Point
Shipping Condition Dry Ice
Research Category Signal Transduction
Substrate Peptide-Based Substrate
Target Entry PDGFRA T674I, PDGFRB, FGFR1, FGFR2, EGFR, FGFR4, FLT1, MUSK, JAK3, FLT4, FGFR3, INSRR, PDGFRA D842V, EPHA2, PDGFRA, PDGFRA V561D
Validated Kinases EGFR, EPHA2, FGFR, FLT1, FLT4, INSRR, JAK3, MUSK, PDGFRA, PDGFRB
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For Research Use Only. Not for use in diagnostic procedures.