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Invitrogen™ Z'-LYTE™ Kinase Assay Kit - Tyrosine 1 Peptide

Catalog No. PV3190
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800 x 20 μL Assays
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PV3190 800 x 20 μL Assays
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Catalog No. PV3190 Supplier Invitrogen™ Supplier No. PV3190
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Description

Z'-LYTE™ Kinase Assay Kit - Tyrosine 1 Peptide

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon an optimal peptide substrate for SRC.

Specifications

Product Line Z´-LYTE
Target Tyrosine Kinases
For Use With (Application) FRET
Assay Tyrosine Kinase Assay
Content And Storage Z´-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.
Assay Entry Biochemical phospho-specific activity (no antibody)
Label or Dye Methylcoumarin
Product Type Kinase Assay Kit
Quantity 800 x 20 μL Assays
Format 384-well plate
Sample Type Src Substrate (EEEIYGEFE)
Excitation/Emission 405⁄465, 535
No. of Reactions 800 Reactions
References Nature, 373, pp. 536-9 (1995)
Readout End Point
Shipping Condition Dry Ice
Substrate Peptide-Based Substrate
Target Entry CSF1R, NTRK2, PTK6, IGF1R, MATK, NTRK3, ROS1, BMX, PTK2B, EPHA3, EPHB4, ERBB4, KDR, NTRK1, SRMS, FRK, ITK, BTK, FES, BLK1, EPHA5
Validated Kinases BLK1, BMX, BTK, CSF1R, EPHA3, EPHA5, EPHB4, ERBB4, FES, FRK, IGF1R, ITK, KDR, MATK, NTRK1, NTRK2, NTRK3, PTK2B, PTK6, ROS1, SRMS
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For Research Use Only. Not for use in diagnostic procedures.