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Thermo Scientific™ Z'-LYTE™ Kinase Assay Kit - Ser/Thr 4 Peptide 
A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format.
Supplier: Thermo Scientific™ PV3177
Description
A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon glycogen synthase, containing Ser-7 and a consensus seq Hyd-X-R-X-X-S/T-X-X, where Hyd is bulky hydrophobic, F > L > V >> A, and X is any residue.
Specifications
| Z'-LYTE™ Kinase Assay Kit - Ser/Thr 4 Peptide, 800 x 20μL Assays, 800 rxns, 405/465nm Excitation, 535nm Emission, Methylcoumarin Label, FRET Technique, Serine/Threonine Kinases Target Enzyme, Peptide-Based Substrate Properties, End Point Readout, Used For Pharma and Biopharma, Protein Assays and Analysis | |
| All necessary reagents for performing primary or secondary screens | |
| Serine/Threonine Kinase Assay | |
| Biochemical phospho-specific activity (no antibody) | |
| 800 x 20 μL Assays | |
| 405/465, 535 nm | |
| Methylcoumarin | |
| End Point | |
| Dry Ice | |
| Peptide-Based Substrate | |
| Serine/Threonine Kinases |
| Z Ì-LYTE | |
| 800 x 20μL | |
| Z'-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions. | |
| Z'-LYTE™ Kinase Assay Kit - Ser/Thr 4 Peptide | |
| 384-well Plate | |
| FRET | |
| 800 Reactions | |
| Biochem J. vol 296, pp. 843-9 (1993), J. Biol Chem, vol. 260, pp 14471-6 (1985) | |
| Ser 7 Glycogen Synthase (KKLNRTLSVA) Based | |
| MAPK8, MAPKAPK2, MAPKAPK5, CAMK2D, MAPK9, STK22B, STK22D, CAMK2A, MAPK14, MAPK10, MAPKAPK3 | |
| MAPKAPK2, MAPKAPK3, MAPKAPK5, STK22B, STK22D, CAMK2A, CAMK2D, MAPK8, MAPK9, MAPK10, MAPK14 |
For Research Use Only. Not for use in diagnostic procedures.