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Thermo Scientific™ Z'-LYTE™ Kinase Assay Kit - Ser/Thr 4 Peptide

Catalog No. PV3177
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800 x 20 μL Assays
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PV3177 800 x 20 μL Assays
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Catalog No. PV3177 Supplier Thermo Scientific™ Supplier No. PV3177
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Description

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format.

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon glycogen synthase, containing Ser-7 and a consensus seq Hyd-X-R-X-X-S/T-X-X, where Hyd is bulky hydrophobic, F > L > V >> A, and X is any residue.

Specifications

Description Z'-LYTE™ Kinase Assay Kit - Ser/Thr 4 Peptide, 800 x 20μL Assays, 800 rxns, 405/465nm Excitation, 535nm Emission, Methylcoumarin Label, FRET Technique, Serine/Threonine Kinases Target Enzyme, Peptide-Based Substrate Properties, End Point Readout, Used For Pharma and Biopharma, Protein Assays and Analysis
Product Line Z ́-LYTE
For Use With (Application) FRET
Includes All necessary reagents for performing primary or secondary screens
Sample Volume 800 x 20μL
Assay Serine/Threonine Kinase Assay
Assay Entry Biochemical phospho-specific activity (no antibody)
Content And Storage Z'-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.
Product Type Z'-LYTE™ Kinase Assay Kit - Ser/Thr 4 Peptide
Quantity 800 x 20 μL Assays
Format 384-well Plate
Excitation/Emission 405/465, 535 nm
Label or Dye Methylcoumarin
No. of Reactions 800 Reactions
Readout End Point
References Biochem J. vol 296, pp. 843-9 (1993), J. Biol Chem, vol. 260, pp 14471-6 (1985)
Shipping Condition Dry Ice
Source Ser 7 Glycogen Synthase (KKLNRTLSVA) Based
Substrate Properties Peptide-Based Substrate
Target Entry MAPK8, MAPKAPK2, MAPKAPK5, CAMK2D, MAPK9, STK22B, STK22D, CAMK2A, MAPK14, MAPK10, MAPKAPK3
Target Enzyme Serine/Threonine Kinases
Validated Kinases MAPKAPK2, MAPKAPK3, MAPKAPK5, STK22B, STK22D, CAMK2A, CAMK2D, MAPK8, MAPK9, MAPK10, MAPK14
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For Research Use Only. Not for use in diagnostic procedures.