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Thermo Scientific™ Z'-LYTE™ Kinase Assay Kit - Ser/Thr 3 Peptide

Catalog No. PV3176
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800 x 20μL, 800 x 20 μL Assays
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PV3176 800 x 20μL, 800 x 20 μL Assays
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Catalog No. PV3176 Supplier Thermo Scientific™ Supplier No. PV3176
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Description

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format.

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon bovine MBP Thr-97 derived peptide, containing a P-X-(S/T)-P consensus sequence, where X is any residue.

Specifications

Description Z'-LYTE™ Kinase Assay Kit - Ser/Thr 3 Peptide, 800 x 20μL Assays, 800 rxns, 405/465nm Excitation, 535nm Emission, Methylcoumarin Label, FRET Technique, Serine/Threonine Kinases Target Enzyme, Peptide-Based Substrate Properties, End Point Readout, Used For Pharma and Biopharma, Protein Assays and Analysis
Product Line Z ́-LYTE
For Use With (Application) Pharma and biopharma, protein assays and analysis, target and lead identification and validation, FRET
Includes All necessary reagents for performing primary or secondary screens
Sample Volume 800 x 20μL
Assay Serine/Threonine Kinase Assay
Assay Entry Biochemical phospho-specific activity (no antibody)
Content And Storage –80°C, Z'-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.
Product Type Z'-LYTE™ Kinase Assay Kit - Ser/Thr 3 Peptide
Quantity 800 x 20μL, 800 x 20 μL Assays
Format 384-well plate
Excitation/Emission 405/465, 535 nm
Label or Dye Methylcoumarin
No. of Reactions 800 x 20 μL Reactions
Readout End Point
References BBRC vol. 61, pp 559-563 (1974), JBC, vol 252, p 4888 (1978)
Shipping Condition Dry Ice
Source Bovine MBP, Thr-97 Derived Peptide S5, Consensus Seq Pro-X-(Ser⁄Thr)-Pro (APRTPGGRR)
Substrate Properties Peptide-Based Substrate
Target Entry MAPK13, MAP2K1, MAP2K6, MAPK1, MAP3K8, BRAF, MAP2K2, MAPK12, MAPK3, BRAF V599E
Target Enzyme Serine/Threonine Kinases
Validated Kinases BRAF, MAPK1, MAPK3, MAPK12, MAPK13, MAP2K1, MAP2K2, MAP3K6, MAP2K8
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For Research Use Only. Not for use in diagnostic procedures.