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Thermo Scientific™ Z'-LYTE™ Kinase Assay Kit - Ser/Thr 3 Peptide 
A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format.
Supplier: Thermo Scientific™ PV3176
Description
A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon bovine MBP Thr-97 derived peptide, containing a P-X-(S/T)-P consensus sequence, where X is any residue.
Specifications
| Z'-LYTE™ Kinase Assay Kit - Ser/Thr 3 Peptide, 800 x 20μL Assays, 800 rxns, 405/465nm Excitation, 535nm Emission, Methylcoumarin Label, FRET Technique, Serine/Threonine Kinases Target Enzyme, Peptide-Based Substrate Properties, End Point Readout, Used For Pharma and Biopharma, Protein Assays and Analysis | |
| All necessary reagents for performing primary or secondary screens | |
| Serine/Threonine Kinase Assay | |
| Biochemical phospho-specific activity (no antibody) | |
| 800 x 20 μL Assays | |
| 405/465, 535 nm | |
| Methylcoumarin | |
| End Point | |
| Dry Ice | |
| Peptide-Based Substrate | |
| Serine/Threonine Kinases |
| Z Ì-LYTE | |
| 800 x 20μL | |
| Z'-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions. | |
| Z'-LYTE™ Kinase Assay Kit - Ser/Thr 3 Peptide | |
| 384-well Plate | |
| FRET | |
| 800 x 20 μL Reactions | |
| BBRC vol. 61, pp 559-563 (1974), JBC, vol 252, p 4888 (1978) | |
| Bovine MBP, Thr-97 Derived Peptide S5, Consensus Seq Pro-X-(SerâThr)-Pro (APRTPGGRR) | |
| MAPK13, MAP2K1, MAP2K6, MAPK1, MAP3K8, BRAF, MAP2K2, MAPK12, MAPK3, BRAF V599E | |
| BRAF, MAPK1, MAPK3, MAPK12, MAPK13, MAP2K1, MAP2K2, MAP3K6, MAP2K8 |
For Research Use Only. Not for use in diagnostic procedures.